Detailed information of TA system TA204046

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 14033..14555
• Replicon: plasmid pSTEC2018_166-c
• Accession: NZ_CP075634
• Organism: Escherichia coli strain 2018-166

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: JFX80_RS26410
• Protein ID: WP_000220566.1
• Coordinates: 14274..14555 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: JFX80_RS26405
• Protein ID: WP_000121744.1
• Coordinates: 14033..14284 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX80_RS26380 (JFX80_26395)	10121..10506	+	386	Protein_12	hypothetical protein	-
JFX80_RS26385 (JFX80_26400)	10506..10610	+	105	Protein_13	ROK family protein	-
JFX80_RS26390 (JFX80_26405)	10754..11467	+	714	WP_024178317.1	oligogalacturonate-specific porin KdgM family protein	-
JFX80_RS26395 (JFX80_26410)	11487..12593	+	1107	WP_062863590.1	N-acetylneuraminate epimerase	-
JFX80_RS26400 (JFX80_26415)	12640..12840	-	201	Protein_16	DNA-binding protein	-
JFX80_RS26405 (JFX80_26420)	14033..14284	+	252	WP_000121744.1	hypothetical protein	Antitoxin
JFX80_RS26410 (JFX80_26425)	14274..14555	+	282	WP_000220566.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JFX80_RS26415 (JFX80_26430)	14809..16021	-	1213	Protein_19	IS91 family transposase	-
JFX80_RS26420 (JFX80_26435)	15904..16356	-	453	WP_215744913.1	hypothetical protein	-
JFX80_RS26425 (JFX80_26440)	16391..16591	-	201	WP_198774796.1	hypothetical protein	-
JFX80_RS26430 (JFX80_26445)	16920..17372	+	453	WP_000670961.1	acyltransferase	-
JFX80_RS26435 (JFX80_26450)	17405..18937	-	1533	WP_032308905.1	NAD(P)-binding domain-containing protein	-
JFX80_RS26440 (JFX80_26455)	18934..19203	-	270	WP_000379710.1	SemiSWEET transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..119130	119130
	-	flank	IS/Tn	-	-	14809..15633	824

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-85)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11070.88 Da
• Isoelectric Point: 10.5430

>T204046 WP_000220566.1 NZ_CP075634:14274-14555 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLSERLENPRVPASRLHGHADRYKIKLRTSGYRLVYQVIDEKVVLLVISVGK
REGNEVYQIADMR

Nucleotide

• Length: 282 bp

>T204046 NZ_CP100520:c4534488-4533955 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCCAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGTATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9222.48 Da
• Isoelectric Point: 4.1683

>AT204046 WP_000121744.1 NZ_CP075634:14033-14284 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVVILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Nucleotide

• Length: 252 bp

>AT204046 NZ_CP100520:c4534765-4534499 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA