Detailed information of TA system TA204045

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 1834..2369
• Replicon: plasmid pSTEC2018_166-c
• Accession: NZ_CP075634
• Organism: Escherichia coli strain 2018-166

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V0VJJ7
• Locus tag: JFX80_RS26335
• Protein ID: WP_000222760.1
• Coordinates: 2082..2369 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0VIP2
• Locus tag: JFX80_RS26330
• Protein ID: WP_001132900.1
• Coordinates: 1834..2085 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX80_RS26325 (JFX80_26340)	1..873	+	873	WP_001536607.1	incFII family plasmid replication initiator RepA	-
JFX80_RS27485	1570..1761	+	192	WP_033810767.1	hypothetical protein	-
JFX80_RS26330 (JFX80_26345)	1834..2085	+	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
JFX80_RS26335 (JFX80_26350)	2082..2369	+	288	WP_000222760.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JFX80_RS26340 (JFX80_26355)	2473..3063	+	591	WP_000977350.1	serine acetyltransferase	-
JFX80_RS26345 (JFX80_26360)	3166..3822	+	657	WP_000536048.1	ProQ/FINO family protein	-
JFX80_RS26350 (JFX80_26365)	3890..4204	+	315	WP_000633742.1	hypothetical protein	-
JFX80_RS26355 (JFX80_26370)	4592..5779	-	1188	WP_215744912.1	IS91 family transposase	-
JFX80_RS26360 (JFX80_26375)	5779..6144	-	366	WP_021521282.1	hypothetical protein	-
JFX80_RS26365 (JFX80_26380)	6491..7190	-	700	Protein_9	transcriptional regulator NanR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..119130	119130
	-	flank	IS/Tn	-	-	4592..5779	1187

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11105.15 Da
• Isoelectric Point: 10.5832

>T204045 WP_000222760.1 NZ_CP075634:2082-2369 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKSIQQQFAKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Nucleotide

• Length: 288 bp

>T204045 NZ_CP100520:3932942-3933160 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9211.41 Da
• Isoelectric Point: 4.0780

>AT204045 WP_001132900.1 NZ_CP075634:1834-2085 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT204045 NZ_CP100520:3932541-3932915 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: V0VJJ7

Antitoxin

• AlphaFold DB: V0VIP2