Detailed information of TA system TA204042

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 108970..109595
• Replicon: plasmid pSTEC2018_166-a
• Accession: NZ_CP075633
• Organism: Escherichia coli strain 2018-166

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JFX80_RS26270
• Protein ID: WP_000911313.1
• Coordinates: 108970..109368 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: JFX80_RS26275
• Protein ID: WP_000450520.1
• Coordinates: 109368..109595 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX80_RS26245 (104091)	104091..104339	+	249	Protein_109	type-F conjugative transfer system secretin TraK	-
JFX80_RS26250 (104337)	104337..105305	+	969	Protein_110	conjugal transfer protein TraG	-
JFX80_RS26255 (105302)	105302..105811	+	510	WP_000628105.1	conjugal transfer entry exclusion protein TraS	-
JFX80_RS26260 (105825)	105825..106556	+	732	WP_000850423.1	conjugal transfer complement resistance protein TraT	-
JFX80_RS26265 (106808)	106808..108961	+	2154	WP_053879280.1	type IV conjugative transfer system coupling protein TraD	-
JFX80_RS26270 (108970)	108970..109368	-	399	WP_000911313.1	type II toxin-antitoxin system VapC family toxin	Toxin
JFX80_RS26275 (109368)	109368..109595	-	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	senB / hlyC / hlyA / hlyB / hlyD / iutA / iucD / iucC / iucB / iucA	1..120601	120601

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T204042 WP_000911313.1 NZ_CP075633:c109368-108970 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T204042 NZ_CP100520:812311-812721 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT204042 WP_000450520.1 NZ_CP075633:c109595-109368 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT204042 NZ_CP100520:812064-812330 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: V0VCB2