Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 82509..83152 Replicon plasmid pSTEC2018_166-a
Accession NZ_CP075633
Organism Escherichia coli strain 2018-166

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag JFX80_RS26090 Protein ID WP_001034046.1
Coordinates 82509..82925 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag JFX80_RS26095 Protein ID WP_001261278.1
Coordinates 82922..83152 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX80_RS26070 (77851) 77851..78765 - 915 WP_000949004.1 iron/manganese ABC transporter substrate-binding protein SitA -
JFX80_RS26075 (79038) 79038..79403 + 366 WP_000091308.1 hypothetical protein -
JFX80_RS26080 (79403) 79403..80590 + 1188 WP_000937604.1 IS91 family transposase -
JFX80_RS26085 (80713) 80713..82464 + 1752 WP_252354386.1 hypothetical protein -
JFX80_RS26090 (82509) 82509..82925 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
JFX80_RS26095 (82922) 82922..83152 - 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
JFX80_RS26100 (83417) 83417..83917 + 501 WP_000528932.1 HEPN family nuclease -
JFX80_RS26105 (83930) 83930..84703 + 774 WP_000905949.1 hypothetical protein -
JFX80_RS26110 (84870) 84870..86003 + 1134 Protein_82 DUF3800 domain-containing protein -
JFX80_RS26115 (86555) 86555..86773 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
JFX80_RS26120 (86775) 86775..87080 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -
JFX80_RS26125 (87081) 87081..87887 + 807 WP_000016982.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD senB / hlyC / hlyA / hlyB / hlyD / iutA / iucD / iucC / iucB / iucA 1..120601 120601
- flank IS/Tn sitABCD - 75316..80590 5274


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T204040 WP_001034046.1 NZ_CP075633:c82925-82509 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T204040 NZ_CP100520:324424-324792 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT204040 WP_001261278.1 NZ_CP075633:c83152-82922 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT204040 NZ_CP100520:324206-324427 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References