Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 59392..60035 Replicon plasmid pSTEC2018_166-a
Accession NZ_CP075633
Organism Escherichia coli strain 2018-166

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag JFX80_RS25990 Protein ID WP_001034044.1
Coordinates 59619..60035 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag JFX80_RS25985 Protein ID WP_001261286.1
Coordinates 59392..59622 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX80_RS25965 (54444) 54444..55076 - 633 Protein_53 RepB family plasmid replication initiator protein -
JFX80_RS25970 (55263) 55263..56450 - 1188 WP_000937604.1 IS91 family transposase -
JFX80_RS25975 (56450) 56450..56815 - 366 WP_000091308.1 hypothetical protein -
JFX80_RS25980 (56897) 56897..59011 - 2115 WP_252354387.1 hypothetical protein -
JFX80_RS25985 (59392) 59392..59622 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
JFX80_RS25990 (59619) 59619..60035 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
JFX80_RS25995 (60110) 60110..61675 + 1566 WP_001128474.1 AAA family ATPase -
JFX80_RS26000 (61660) 61660..62682 + 1023 WP_000361402.1 helicase UvrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD senB / hlyC / hlyA / hlyB / hlyD / iutA / iucD / iucC / iucB / iucA 1..120601 120601
- flank IS/Tn - - 62936..63439 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T204039 WP_001034044.1 NZ_CP075633:59619-60035 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T204039 NZ_CP100515:c5249189-5248806 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT204039 WP_001261286.1 NZ_CP075633:59392-59622 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT204039 NZ_CP100515:c5249431-5249189 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATACCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References