Detailed information of TA system TA204036

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4914376..4915001
• Replicon: chromosome
• Accession: NZ_CP075632
• Organism: Escherichia coli strain 2018-166

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JFX80_RS24315
• Protein ID: WP_000911330.1
• Coordinates: 4914603..4915001 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: JFX80_RS24310
• Protein ID: WP_000450524.1
• Coordinates: 4914376..4914603 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX80_RS24285 (4910179)	4910179..4910649	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
JFX80_RS24290 (4910649)	4910649..4911221	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
JFX80_RS24295 (4911367)	4911367..4912245	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
JFX80_RS24300 (4912262)	4912262..4913296	+	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
JFX80_RS24305 (4913509)	4913509..4914222	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
JFX80_RS24310 (4914376)	4914376..4914603	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
JFX80_RS24315 (4914603)	4914603..4915001	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
JFX80_RS24320 (4915148)	4915148..4916011	+	864	WP_001267498.1	neutral zinc metallopeptidase	-
JFX80_RS24325 (4916026)	4916026..4918041	+	2016	WP_000829394.1	tRNA cytosine(34) acetyltransferase TmcA	-
JFX80_RS24330 (4918115)	4918115..4918813	+	699	WP_000679812.1	esterase	-
JFX80_RS24335 (4918923)	4918923..4919123	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T204036 WP_000911330.1 NZ_CP075632:4914603-4915001 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T204036 NZ_CP100515:c4494230-4493820 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT204036 WP_000450524.1 NZ_CP075632:4914376-4914603 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT204036 NZ_CP100515:c4494477-4494211 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33