Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 4582649..4583232 Replicon chromosome
Accession NZ_CP075632
Organism Escherichia coli strain 2018-166

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag JFX80_RS22605 Protein ID WP_000254745.1
Coordinates 4582897..4583232 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag JFX80_RS22600 Protein ID WP_000581937.1
Coordinates 4582649..4582897 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX80_RS22590 (4578988) 4578988..4580289 + 1302 WP_000046800.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
JFX80_RS22595 (4580337) 4580337..4582571 + 2235 WP_000226815.1 GTP pyrophosphokinase -
JFX80_RS22600 (4582649) 4582649..4582897 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
JFX80_RS22605 (4582897) 4582897..4583232 + 336 WP_000254745.1 endoribonuclease MazF Toxin
JFX80_RS22610 (4583303) 4583303..4584094 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
JFX80_RS22615 (4584322) 4584322..4585959 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
JFX80_RS22620 (4586047) 4586047..4587345 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T204035 WP_000254745.1 NZ_CP075632:4582897-4583232 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T204035 NZ_CP100515:c3431159-3431057 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT204035 WP_000581937.1 NZ_CP075632:4582649-4582897 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT204035 NZ_CP100515:3431050-3431195 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References