Detailed information of TA system TA204032

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 3823888..3824474
• Replicon: chromosome
• Accession: NZ_CP075632
• Organism: Escherichia coli strain 2018-166

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0H0RQZ4
• Locus tag: JFX80_RS18775
• Protein ID: WP_042014162.1
• Coordinates: 3823888..3824118 (+)
• Length: 77 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JFX80_RS18780
• Protein ID: WP_000593552.1
• Coordinates: 3824115..3824474 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX80_RS18765 (3819925)	3819925..3822660	+	2736	WP_000149132.1	ribosome-associated ATPase/putative transporter RbbA	-
JFX80_RS18770 (3822660)	3822660..3823784	+	1125	WP_001216257.1	ABC transporter permease	-
JFX80_RS18775 (3823888)	3823888..3824118	+	231	WP_042014162.1	type II toxin-antitoxin system HicA family toxin	Toxin
JFX80_RS18780 (3824115)	3824115..3824474	+	360	WP_000593552.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
JFX80_RS18785 (3824594)	3824594..3824995	-	402	WP_001190062.1	nickel-responsive transcriptional regulator NikR	-
JFX80_RS18790 (3825001)	3825001..3825807	-	807	WP_000173666.1	nickel import ATP-binding protein NikE	-
JFX80_RS18795 (3825804)	3825804..3826568	-	765	WP_001136236.1	nickel import ATP-binding protein NikD	-
JFX80_RS18800 (3826568)	3826568..3827401	-	834	WP_001008963.1	nickel ABC transporter permease subunit NikC	-
JFX80_RS18805 (3827398)	3827398..3828342	-	945	WP_000947068.1	nickel ABC transporter permease subunit NikB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (15-65)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 77 a.a.
• Molecular weight: 8419.79 Da
• Isoelectric Point: 8.5518

>T204032 WP_042014162.1 NZ_CP075632:3823888-3824118 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Nucleotide

• Length: 231 bp

>T204032 NZ_CP100515:642019-642552 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTTTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13462.37 Da
• Isoelectric Point: 5.1987

>AT204032 WP_000593552.1 NZ_CP075632:3824115-3824474 [Escherichia coli]
MIKLKTPNFMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT204032 NZ_CP100515:641742-642008 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: A0A0H0RQZ4