Detailed information of TA system TA204028

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2993767..2994565
• Replicon: chromosome
• Accession: NZ_CP075632
• Organism: Escherichia coli strain 2018-166

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0P7PH46
• Locus tag: JFX80_RS14940
• Protein ID: WP_000854896.1
• Coordinates: 2994188..2994565 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A234XUW5
• Locus tag: JFX80_RS14935
• Protein ID: WP_024175300.1
• Coordinates: 2993767..2994141 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX80_RS14910 (2991111)	2991111..2991344	+	234	WP_001117568.1	DUF905 family protein	-
JFX80_RS14915 (2991435)	2991435..2992253	+	819	WP_001234751.1	DUF932 domain-containing protein	-
JFX80_RS14920 (2992343)	2992343..2992828	+	486	WP_000214386.1	antirestriction protein	-
JFX80_RS14925 (2992844)	2992844..2993320	+	477	WP_001186727.1	RadC family protein	-
JFX80_RS14930 (2993383)	2993383..2993604	+	222	WP_000692358.1	DUF987 domain-containing protein	-
JFX80_RS14935 (2993767)	2993767..2994141	+	375	WP_024175300.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JFX80_RS14940 (2994188)	2994188..2994565	+	378	WP_000854896.1	TA system toxin CbtA family protein	Toxin
JFX80_RS14945 (2994562)	2994562..2995050	+	489	WP_000761662.1	DUF5983 family protein	-
JFX80_RS14950 (2995062)	2995062..2995259	+	198	WP_000839256.1	DUF957 domain-containing protein	-
JFX80_RS14955 (2995344)	2995344..2996210	+	867	WP_000014511.1	DUF4942 domain-containing protein	-
JFX80_RS14960 (2996282)	2996282..2996545	+	264	WP_001143297.1	type II toxin-antitoxin system ParD family antitoxin	-
JFX80_RS14965 (2996542)	2996542..2996868	+	327	WP_000779483.1	type II toxin-antitoxin system RelE/ParE family toxin	-
JFX80_RS27600 (2997331)	2997331..2997490	+	160	Protein_2952	integrase	-
JFX80_RS14970 (2997750)	2997750..2999288	+	1539	WP_001187182.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14210.19 Da
• Isoelectric Point: 7.2920

>T204028 WP_000854896.1 NZ_CP075632:2994188-2994565 [Escherichia coli]
MKTLSDTHVREASRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRMVNNITQGKHPEAQQ

Nucleotide

• Length: 378 bp

>T204028 NZ_CP100515:326481-326849 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGTCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13664.42 Da
• Isoelectric Point: 6.4757

>AT204028 WP_024175300.1 NZ_CP075632:2993767-2994141 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVKPCFGARLVQEGNRLHYLADRAGISGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHSVTLYAKGLTCEADTLGSCGYVYLTVYPTPATATTS

Nucleotide

• Length: 375 bp

>AT204028 NZ_CP100515:326263-326484 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGCTCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A0P7PH46

Antitoxin

• AlphaFold DB: A0A234XUW5