Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2086915..2087533 Replicon chromosome
Accession NZ_CP075632
Organism Escherichia coli strain 2018-166

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JFX80_RS10495 Protein ID WP_001291435.1
Coordinates 2087315..2087533 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JFX80_RS10490 Protein ID WP_000344800.1
Coordinates 2086915..2087289 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX80_RS10480 (2082004) 2082004..2083197 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JFX80_RS10485 (2083220) 2083220..2086369 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
JFX80_RS10490 (2086915) 2086915..2087289 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JFX80_RS10495 (2087315) 2087315..2087533 + 219 WP_001291435.1 HHA domain-containing protein Toxin
JFX80_RS10500 (2087705) 2087705..2088256 + 552 WP_000102564.1 maltose O-acetyltransferase -
JFX80_RS10505 (2088372) 2088372..2088842 + 471 WP_000136192.1 YlaC family protein -
JFX80_RS10510 (2089006) 2089006..2090556 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JFX80_RS10515 (2090598) 2090598..2090951 - 354 WP_000878141.1 DUF1428 family protein -
JFX80_RS10525 (2091330) 2091330..2091641 + 312 WP_000409911.1 MGMT family protein -
JFX80_RS10530 (2091672) 2091672..2092244 - 573 WP_000779827.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T204024 WP_001291435.1 NZ_CP075632:2087315-2087533 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T204024 NZ_CP100511:c4560375-4559890 [Klebsiella pneumoniae]
ATGATCTTGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTAGTTAAT
GCCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT204024 WP_000344800.1 NZ_CP075632:2086915-2087289 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT204024 NZ_CP100511:c4560665-4560372 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References