Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 1477864..1478453 Replicon chromosome
Accession NZ_CP075632
Organism Escherichia coli strain 2018-166

Toxin (Protein)


Gene name hicA Uniprot ID A0A398TMA1
Locus tag JFX80_RS07570 Protein ID WP_000513533.1
Coordinates 1478205..1478453 (-) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A3J8R0E8
Locus tag JFX80_RS07565 Protein ID WP_000681611.1
Coordinates 1477864..1478208 (-) Length 115 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX80_RS07530 (1474315) 1474315..1474692 - 378 WP_001271433.1 hypothetical protein -
JFX80_RS27235 (1474900) 1474900..1475169 - 270 WP_001115081.1 hypothetical protein -
JFX80_RS07540 (1475224) 1475224..1475427 - 204 WP_001240876.1 Cro/CI family transcriptional regulator -
JFX80_RS07545 (1475523) 1475523..1476236 + 714 WP_001056249.1 LexA family transcriptional regulator -
JFX80_RS07550 (1476333) 1476333..1476494 + 162 WP_071525586.1 transmembrane anchored protein -
JFX80_RS07555 (1476674) 1476674..1477576 + 903 WP_000081325.1 serine dehydrogenasease -
JFX80_RS07560 (1477577) 1477577..1477747 + 171 WP_167580584.1 hypothetical protein -
JFX80_RS07565 (1477864) 1477864..1478208 - 345 WP_000681611.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
JFX80_RS07570 (1478205) 1478205..1478453 - 249 WP_000513533.1 type II toxin-antitoxin system HicA family toxin Toxin
JFX80_RS07575 (1479217) 1479217..1479987 + 771 WP_000529245.1 Rha family transcriptional regulator -
JFX80_RS07580 (1480058) 1480058..1480270 + 213 WP_000917251.1 hypothetical protein -
JFX80_RS07585 (1480282) 1480282..1480563 + 282 WP_000934192.1 hypothetical protein -
JFX80_RS07590 (1480584) 1480584..1480865 + 282 WP_000995348.1 host nuclease inhibitor GamL -
JFX80_RS07595 (1480883) 1480883..1480966 + 84 Protein_1502 recombinase RecT -
JFX80_RS07600 (1481023) 1481023..1481720 + 698 WP_094096600.1 IS1-like element IS1A family transposase -
JFX80_RS07605 (1481804) 1481804..1482088 + 285 WP_001208773.1 excisionase family protein -
JFX80_RS07610 (1482141) 1482141..1483451 + 1311 WP_001368133.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - stxB / stxA 1370224..1487244 117020


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9436.07 Da        Isoelectric Point: 10.3195

>T204023 WP_000513533.1 NZ_CP075632:c1478453-1478205 [Escherichia coli]
MGKADKLLEKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL

Download         Length: 249 bp

>T204023 NZ_CP100511:c4493594-4493184 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 115 a.a.        Molecular weight: 13065.80 Da        Isoelectric Point: 4.6791

>AT204023 WP_000681611.1 NZ_CP075632:c1478208-1477864 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEASTLAELEQEFKTSVELYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVNHHTGA

Download         Length: 345 bp

>AT204023 NZ_CP100511:c4493841-4493575 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A398TMA1

Antitoxin

Source ID Structure
AlphaFold DB A0A3J8R0E8

References