Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 930583..931221 Replicon chromosome
Accession NZ_CP075632
Organism Escherichia coli strain 2018-166

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag JFX80_RS04650 Protein ID WP_000813794.1
Coordinates 931045..931221 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag JFX80_RS04645 Protein ID WP_001270286.1
Coordinates 930583..930999 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX80_RS04625 (925736) 925736..926676 - 941 Protein_916 ABC transporter permease -
JFX80_RS04630 (926677) 926677..927690 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
JFX80_RS04635 (927708) 927708..928853 - 1146 WP_000047456.1 ABC transporter substrate-binding protein -
JFX80_RS04640 (929098) 929098..930504 - 1407 WP_000760592.1 PLP-dependent aminotransferase family protein -
JFX80_RS04645 (930583) 930583..930999 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
JFX80_RS04650 (931045) 931045..931221 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
JFX80_RS04655 (931443) 931443..931673 + 231 WP_000494244.1 YncJ family protein -
JFX80_RS04660 (931765) 931765..933726 - 1962 WP_024178299.1 23S rRNA 5-hydroxycytidine C2501 synthase -
JFX80_RS04665 (933799) 933799..934335 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
JFX80_RS04670 (934427) 934427..935608 + 1182 WP_032308624.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 935648..936913 1265


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T204022 WP_000813794.1 NZ_CP075632:c931221-931045 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T204022 NZ_CP100511:c3430419-3430317 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT204022 WP_001270286.1 NZ_CP075632:c930999-930583 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT204022 NZ_CP100511:3430310-3430455 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References