Detailed information of TA system TA204007

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 51326..51951
• Replicon: plasmid pP15-385-a
• Accession: NZ_CP075628
• Organism: Escherichia coli strain P15-385

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JFX93_RS24340
• Protein ID: WP_000911317.1
• Coordinates: 51553..51951 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L4J1D2
• Locus tag: JFX93_RS24335
• Protein ID: WP_000450532.1
• Coordinates: 51326..51553 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX93_RS24335 (51326)	51326..51553	+	228	WP_000450532.1	toxin-antitoxin system antitoxin VapB	Antitoxin
JFX93_RS24340 (51553)	51553..51951	+	399	WP_000911317.1	type II toxin-antitoxin system VapC family toxin	Toxin
JFX93_RS24345 (51960)	51960..52592	-	633	Protein_53	type IV secretion system DNA-binding domain-containing protein	-
JFX93_RS24350 (52655)	52655..53811	+	1157	WP_099205956.1	IS3-like element IS600 family transposase	-
JFX93_RS24355 (53858)	53858..55009	+	1152	WP_072158966.1	conjugal transfer protein TraW	-
JFX93_RS24360 (55006)	55006..55575	+	570	WP_000650755.1	conjugal transfer protein TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyA / hlyC	1..118360	118360

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14857.14 Da
• Isoelectric Point: 8.5264

>T204007 WP_000911317.1 NZ_CP075628:51553-51951 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Nucleotide

• Length: 399 bp

>T204007 NZ_CP100501:2076-2363 [Escherichia coli]
ATGACTTATACAGTAAAATTCAGGGATGATGCGCTCAAAGAATGGCTGAAACTGGATAAATCCATTCAACAGCAGTTTGC
GAAAAAACTAAAGAAATGCAGCGAAAATCCTCATATTCCTTCCGCAAAACTGCGTGGATTAAAAGACTGCTACAAAATCA
AACTTCGCGCATCGGGGTTTCGTCTGGTCTATCAGGTTATTGATGACATGCTAATTATTGCGGTTGTCGCGGTAGGTAAA
CGTGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8512.55 Da
• Isoelectric Point: 4.5358

>AT204007 WP_000450532.1 NZ_CP075628:51326-51553 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT204007 NZ_CP100501:1828-2079 [Escherichia coli]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTATCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCGGAACTCTATGAAAGGATGC
TTGATGCGCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Structures

Antitoxin

• AlphaFold DB: A0A829CHF5