Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4465955..4466586 Replicon chromosome
Accession NZ_CP075627
Organism Escherichia coli strain P15-385

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag JFX93_RS21750 Protein ID WP_001259388.1
Coordinates 4466311..4466586 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag JFX93_RS21745 Protein ID WP_000593555.1
Coordinates 4465955..4466314 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX93_RS21720 (4462087) 4462087..4463031 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
JFX93_RS21725 (4463028) 4463028..4463861 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
JFX93_RS21730 (4463861) 4463861..4464625 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
JFX93_RS21735 (4464622) 4464622..4465428 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
JFX93_RS21740 (4465434) 4465434..4465835 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
JFX93_RS21745 (4465955) 4465955..4466314 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
JFX93_RS21750 (4466311) 4466311..4466586 - 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
JFX93_RS21755 (4466659) 4466659..4467783 - 1125 WP_001314210.1 ABC transporter permease -
JFX93_RS21760 (4467783) 4467783..4470518 - 2736 WP_000149125.1 ribosome-associated ATPase/putative transporter RbbA -
JFX93_RS21765 (4470515) 4470515..4471582 - 1068 WP_000361483.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T204001 WP_001259388.1 NZ_CP075627:c4466586-4466311 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T204001 NZ_CP100500:c4624207-4623893 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTATCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT204001 WP_000593555.1 NZ_CP075627:c4466314-4465955 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT204001 NZ_CP100500:c4624443-4624210 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References