Detailed information of TA system TA020400

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-AbrB
• Location: 1340396..1340996
• Replicon: chromosome
• Accession: NC_002754
• Organism: Sulfolobus solfataricus P2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: SSO_RS07250
• Protein ID: WP_048054229.1
• Coordinates: 1340607..1340996 (+)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A157T1M9
• Locus tag: SSO_RS07245
• Protein ID: WP_048054328.1
• Coordinates: 1340396..1340620 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SSO_RS07220 (SSO1478)	1336464..1336907	-	444	WP_010923418.1	hypothetical protein	-
SSO_RS07225 (SSO1479)	1336991..1337419	-	429	WP_010923419.1	transposase	-
SSO_RS17475	1337487..1337657	+	171	Protein_1427	transposase	-
SSO_RS07230 (SSO1480)	1337632..1338528	-	897	Protein_1428	ISH3-like element ISC1225 family transposase	-
SSO_RS07235 (SSO1481)	1338701..1339660	-	960	WP_010923364.1	IS5-like element ISC1234 family transposase	-
SSO_RS07240 (SSO8189)	1339758..1339922	-	165	Protein_1430	DUF4322 domain-containing protein	-
SSO_RS07245	1340396..1340620	+	225	WP_048054328.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
SSO_RS07250 (SSO1483)	1340607..1340996	+	390	WP_048054229.1	PIN domain-containing protein	Toxin
SSO_RS07255 (SSO1484)	1341997..1342947	+	951	WP_010923426.1	IS110-like element ISC1190 family transposase	-
SSO_RS07260 (SSO1486)	1343008..1343346	-	339	WP_010923427.1	hypothetical protein	-
SSO_RS07265 (SSO1487)	1343471..1344444	+	974	Protein_1435	IS5 family transposase	-
SSO_RS07270 (SSO1489)	1344586..1345083	+	498	WP_014511660.1	hypothetical protein	-
SSO_RS17025	1345479..1345624	+	146	Protein_1437	IS630 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1312127..1346141	34014
	-	inside	IScluster/Tn	-	-	1333044..1352472	19428

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-121)

Antitoxin

• SpoVT-AbrB domain (4-45)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14446.03 Da
• Isoelectric Point: 4.5625

>T20400 WP_048054229.1 NC_002754:1340607-1340996 [Saccharolobus solfataricus P2]
MIVVDTNVLIYATLEDSEFHTQSLEIIEGSDIIVPQIVVFEYIKVLSEIVQNLDFIKTKISELNNFVVVCEDLNTIALAL
RLLAELKLSLKDINDMIILTAAIKTNSSIATFDQKLRKIADKKGVKVLP

Nucleotide

• Length: 390 bp

>T20400 NC_002754:1340607-1340996 [Saccharolobus solfataricus P2]
ATGATAGTAGTTGATACGAATGTCCTAATTTACGCCACTCTTGAAGACAGCGAATTTCATACTCAAAGTCTAGAGATAAT
TGAGGGATCAGACATAATAGTTCCCCAAATTGTAGTCTTCGAATACATAAAGGTACTATCTGAAATTGTACAGAACTTAG
ATTTCATAAAAACAAAAATCTCAGAGTTAAACAATTTTGTCGTTGTTTGCGAAGACCTTAATACTATAGCACTAGCGTTG
AGACTATTAGCAGAGTTAAAACTTTCACTGAAAGACATTAACGATATGATAATCTTAACCGCAGCAATTAAGACAAACAG
CTCCATAGCCACATTTGATCAGAAGCTTAGAAAGATTGCAGACAAGAAGGGAGTTAAAGTTTTGCCTTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8530.93 Da
• Isoelectric Point: 8.5049

>AT20400 WP_048054328.1 NC_002754:1340396-1340620 [Saccharolobus solfataricus P2]
MISKVTRNFQVTIPYEIRKVLGIKEGDYIEFAIENGKVMIKPVRKVWSTIRLGREVTVEEIEEIASKAFKDDSS

Nucleotide

• Length: 225 bp

>AT20400 NC_002754:1340396-1340620 [Saccharolobus solfataricus P2]
ATGATATCTAAGGTCACCAGGAACTTTCAAGTCACAATTCCTTATGAGATTAGAAAAGTGCTAGGAATAAAAGAGGGAGA
CTATATTGAGTTCGCAATAGAAAACGGTAAGGTCATGATAAAACCTGTTAGAAAAGTTTGGTCAACTATTAGACTTGGAA
GAGAAGTTACAGTGGAGGAGATTGAGGAAATCGCAAGTAAGGCCTTTAAAGATGATAGTAGTTGA

Structures

Antitoxin

• AlphaFold DB: A0A157T1M9