Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3782638..3783292 Replicon chromosome
Accession NZ_CP075627
Organism Escherichia coli strain P15-385

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag JFX93_RS18375 Protein ID WP_000244777.1
Coordinates 3782638..3783045 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag JFX93_RS18380 Protein ID WP_000354046.1
Coordinates 3783026..3783292 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX93_RS18355 (3778595) 3778595..3780328 - 1734 WP_000813223.1 single-stranded-DNA-specific exonuclease RecJ -
JFX93_RS18360 (3780334) 3780334..3781044 - 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
JFX93_RS18365 (3781069) 3781069..3781965 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
JFX93_RS18370 (3782077) 3782077..3782598 + 522 WP_001055874.1 flavodoxin FldB -
JFX93_RS18375 (3782638) 3782638..3783045 - 408 WP_000244777.1 protein YgfX Toxin
JFX93_RS18380 (3783026) 3783026..3783292 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
JFX93_RS18385 (3783535) 3783535..3784515 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
JFX93_RS18390 (3784711) 3784711..3785370 - 660 WP_000250274.1 hemolysin III family protein -
JFX93_RS18395 (3785534) 3785534..3785845 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
JFX93_RS18400 (3785890) 3785890..3787323 + 1434 WP_198792973.1 6-phospho-beta-glucosidase BglA -
JFX93_RS18405 (3787380) 3787380..3788123 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T203998 WP_000244777.1 NZ_CP075627:c3783045-3782638 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T203998 NZ_CP100500:c3527750-3527373 [Escherichia coli]
ATGAAAACATTCCCCGACACACACGTACGGGCAACATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGGCTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCGGGTATTTCACTGTGTGATGCGGTGAACTTTCTTGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCAGGAGCTTCATCGCAGTTAATCAGCAGCATTGATATTCTCCGGGCCCGCCGGGCGACCGGCCTGATGACCCGCAG
CCACTACAGAATGGTGAACAACATCACCCTGGGTAAACACTGTGAGGTGAAACCATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT203998 WP_000354046.1 NZ_CP075627:c3783292-3783026 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT203998 NZ_CP100500:c3528111-3527809 [Escherichia coli]
CTGCCCTGCACCGTGACACCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCAGACCGCGC
CGGTATCAGAGGCCGGTTCAGCGACGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAGCTGGAAC
TCATGCTCACCAGCGGTGAACTCAGTCCCCGCCATCAGCATACCGTGACACTGTATGCGAAAGGGCTGACCTGCGATGCC
GACACCCTCGGCAGTTGTGGTTACGTGTATATCGCCATTTATCCGACACCGGCAACACCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References