Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3646426..3647009 Replicon chromosome
Accession NZ_CP075627
Organism Escherichia coli strain P15-385

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag JFX93_RS17760 Protein ID WP_000254745.1
Coordinates 3646426..3646761 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag JFX93_RS17765 Protein ID WP_000581937.1
Coordinates 3646761..3647009 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX93_RS17745 (3642313) 3642313..3643611 - 1299 WP_000036723.1 phosphopyruvate hydratase -
JFX93_RS17750 (3643699) 3643699..3645336 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
JFX93_RS17755 (3645564) 3645564..3646355 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
JFX93_RS17760 (3646426) 3646426..3646761 - 336 WP_000254745.1 endoribonuclease MazF Toxin
JFX93_RS17765 (3646761) 3646761..3647009 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
JFX93_RS17770 (3647087) 3647087..3649321 - 2235 WP_000226815.1 GTP pyrophosphokinase -
JFX93_RS17775 (3649369) 3649369..3650670 - 1302 WP_000046791.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T203997 WP_000254745.1 NZ_CP075627:c3646761-3646426 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T203997 NZ_CP100500:3108222-3108329 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT203997 WP_000581937.1 NZ_CP075627:c3647009-3646761 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT203997 NZ_CP100500:c3108169-3108108 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References