Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1030296..1030914 Replicon chromosome
Accession NZ_CP075627
Organism Escherichia coli strain P15-385

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JFX93_RS04790 Protein ID WP_001291435.1
Coordinates 1030296..1030514 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JFX93_RS04795 Protein ID WP_000344800.1
Coordinates 1030540..1030914 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JFX93_RS04755 (1025586) 1025586..1026158 + 573 WP_000779833.1 YbaY family lipoprotein -
JFX93_RS04760 (1026189) 1026189..1026500 - 312 WP_000409911.1 MGMT family protein -
JFX93_RS04770 (1026879) 1026879..1027232 + 354 WP_000878141.1 DUF1428 family protein -
JFX93_RS04775 (1027274) 1027274..1028824 - 1551 WP_198792985.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JFX93_RS04780 (1028988) 1028988..1029458 - 471 WP_000136192.1 YlaC family protein -
JFX93_RS04785 (1029574) 1029574..1030125 - 552 WP_000102564.1 maltose O-acetyltransferase -
JFX93_RS04790 (1030296) 1030296..1030514 - 219 WP_001291435.1 HHA domain-containing protein Toxin
JFX93_RS04795 (1030540) 1030540..1030914 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JFX93_RS04800 (1031460) 1031460..1034609 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
JFX93_RS04805 (1034632) 1034632..1035825 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T203985 WP_001291435.1 NZ_CP075627:c1030514-1030296 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T203985 NZ_CP100500:c865409-865035 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCAAGCAAGCGGCCAGTTCACGCCCGTCTCCGCTTGAAATCTGGCAGATACTGCTGAC
CCGGCTGCTGGACCAGCATTACGGCCTCACACTGCATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT203985 WP_000344800.1 NZ_CP075627:c1030914-1030540 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT203985 NZ_CP100500:c865817-865455 [Escherichia coli]
GTGTCAGACACACTCCCCGACGACAATCACGACCGCCCCTGGTGGGGACTACCCTGCACCGTGACGCCCTGTTTCGGGGC
CTGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCTTCAGCGACGCAGATG
CATACCATCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGCGGTGAACTCAGTCCCCGT
CATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTGTGGCTACGTTTATAT
GGCTGTTTATCCGACACCAGCAGTTCCCGCAACAACAGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References