Detailed information of TA system TA203984

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 996000..996837
• Replicon: chromosome
• Accession: NZ_CP075627
• Organism: Escherichia coli strain P15-385

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: JFX93_RS04620
• Protein ID: WP_000227784.1
• Coordinates: 996000..996542 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: JFX93_RS04625
• Protein ID: WP_001297137.1
• Coordinates: 996526..996837 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JFX93_RS04600 (991539)	991539..992450	-	912	WP_000705874.1	2-dehydropantoate 2-reductase	-
JFX93_RS04605 (992618)	992618..993109	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
JFX93_RS04610 (993237)	993237..994601	-	1365	WP_001000974.1	MFS transporter	-
JFX93_RS04615 (995009)	995009..995944	+	936	WP_001365761.1	tetratricopeptide repeat protein	-
JFX93_RS04620 (996000)	996000..996542	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
JFX93_RS04625 (996526)	996526..996837	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
JFX93_RS04630 (997022)	997022..997912	-	891	WP_000971336.1	heme o synthase	-
JFX93_RS04635 (997924)	997924..998253	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
JFX93_RS04640 (998253)	998253..998867	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
JFX93_RS04645 (998857)	998857..1000848	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
JFX93_RS04650 (1000870)	1000870..1001817	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T203984 WP_000227784.1 NZ_CP075627:c996542-996000 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T203984 NZ_CP100500:c665672-665208 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACTCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT203984 WP_001297137.1 NZ_CP075627:c996837-996526 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT203984 NZ_CP100500:c666007-665672 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCTTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3