Detailed information of TA system TA203976

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 56568..57237
• Replicon: plasmid p1207_1
• Accession: NZ_CP075605
• Organism: Enterococcus faecalis strain 1207/14

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: KKP64_RS16585
• Protein ID: WP_010708525.1
• Coordinates: 56842..57237 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: Q9AL01
• Locus tag: KKP64_RS16580
• Protein ID: WP_002331065.1
• Coordinates: 56568..56840 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KKP64_RS16550 (KKP64_16560)	51867..52775	+	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
KKP64_RS16555 (KKP64_16565)	52772..53314	+	543	WP_000627290.1	streptothricin N-acetyltransferase Sat4	-
KKP64_RS16560 (KKP64_16570)	53407..54201	+	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
KKP64_RS16565 (KKP64_16575)	54477..55049	+	573	WP_001079938.1	HTH domain-containing protein	-
KKP64_RS16570 (KKP64_16580)	55347..56243	+	897	WP_002334978.1	AAA family ATPase	-
KKP64_RS16575 (KKP64_16585)	56342..56551	+	210	WP_000527318.1	peptide-binding protein	-
KKP64_RS16580 (KKP64_16590)	56568..56840	+	273	WP_002331065.1	antitoxin	Antitoxin
KKP64_RS16585 (KKP64_16595)	56842..57237	+	396	WP_010708525.1	zeta toxin family protein	Toxin
KKP64_RS16590 (KKP64_16600)	57265..57945	+	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
KKP64_RS16595 (KKP64_16605)	57975..58676	-	702	WP_216788178.1	replication initiation protein	-
KKP64_RS16600 (KKP64_16610)	59301..59387	+	87	WP_107196852.1	type I toxin-antitoxin system Fst family toxin	-
KKP64_RS16605 (KKP64_16615)	59906..60106	-	201	WP_002364281.1	hypothetical protein	-
KKP64_RS16610 (KKP64_16620)	60195..61874	-	1680	WP_010717414.1	MobV family relaxase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	ant(6)-Ia / aph(3')-III / erm(B) / cat(pC221)	-	1..75084	75084
	-	inside	IScluster/Tn	ant(6)-Ia / aph(3')-III	-	37118..57945	20827

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (28-118)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14867.86 Da
• Isoelectric Point: 5.2505

>T203976 WP_010708525.1 NZ_CP075605:56842-57237 [Enterococcus faecalis]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEALISRLSDQGYNLVIEGFCCKVLNLLSNKVE

Nucleotide

• Length: 396 bp

>T203976 NZ_CP100498:20574-21056 [Klebsiella michiganensis]
ATGGGAATGAGAGCGCCTGAATCCCTGACGGCTGAACATAACATCGCTGAGTTCTGCTGCCAGGAGCCTGCCCTTAATGA
GTGGTTAAAGAAGAAGGCTCTCAGAAACCACAGTACCGGCATTTCCCGTGTCTATGTCGTCTGCGCAGAAAACTCGAACC
GGGTCATCGGTTATTATTGTTTATCTTCGGGTAGCGTCCACCGTAACACAGTGCCTGGGGCCTATAGACGCAACGCGCCT
GAAGTCATTCCGGTCATTGTCCTGGGACGGCTTGCCATCGACCAGGCCTGGTCCGGGAATGGGCTTGGTGCCGCACTTCT
TAAGGATGCTATATACCGTACGCAAAACATTGCCTTTCAGGTCGGGGTGAGAGCACTGGCCGTGCATGCGCTGAATGAAG
AAGTTAAATGTTTTTACACCCGTTTTGGCTTTGAGCCTTCCATAGTCAACACGCTGACATTGTTATTCCCGATAAAAGTC
TGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10733.17 Da
• Isoelectric Point: 4.4865

>AT203976 WP_002331065.1 NZ_CP075605:56568-56840 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILNKEKVA

Nucleotide

• Length: 273 bp

>AT203976 NZ_CP100498:20305-20583 [Klebsiella michiganensis]
ATGAAACCAGACGTTCGTGAAGCTCCGATCAATATCCGGGCCAAAACATCACAGCGTGACCTGATCGACATGGCCGCAAG
CCTGGTCTCAAAATCACGGACTGATTTTATGCTTGAGGTTGCCTGCAGGGAAGCACAGGACATTCTACTTGATCAACGAC
TGTTCCTCCTCAATGACAATCAGTTTGAGGCGTTTATTGAAGAGCTTGACGCCCCAATAACGCCGGAGCGCCAGGCGCGA
ATCGATAACCTGATGAACCGCAAATCACCATGGGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A828ZL55