Detailed information of TA system TA203975

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-DinJ
• Location: 27623..28157
• Replicon: plasmid p1207_1
• Accession: NZ_CP075605
• Organism: Enterococcus faecalis strain 1207/14

Toxin (Protein)

• Gene name: relE
• Uniprot ID: R3JX52
• Locus tag: KKP64_RS16395
• Protein ID: WP_002360769.1
• Coordinates: 27882..28157 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: R3H6P0
• Locus tag: KKP64_RS16390
• Protein ID: WP_002369771.1
• Coordinates: 27623..27889 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KKP64_RS16360 (KKP64_16370)	23726..24511	+	786	WP_000803546.1	winged helix-turn-helix domain-containing protein	-
KKP64_RS16365 (KKP64_16375)	24517..25005	+	489	WP_002368088.1	hypothetical protein	-
KKP64_RS16370 (KKP64_16380)	25597..25863	+	267	WP_002368091.1	hypothetical protein	-
KKP64_RS16375 (KKP64_16385)	25896..26390	+	495	WP_002368092.1	DnaJ domain-containing protein	-
KKP64_RS16380 (KKP64_16390)	26488..26904	+	417	WP_002368093.1	single-stranded DNA-binding protein	-
KKP64_RS16385 (KKP64_16395)	26928..27512	+	585	WP_002368095.1	thermonuclease family protein	-
KKP64_RS16390 (KKP64_16400)	27623..27889	+	267	WP_002369771.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
KKP64_RS16395 (KKP64_16405)	27882..28157	+	276	WP_002360769.1	type II toxin-antitoxin system YafQ family toxin	Toxin
KKP64_RS16400 (KKP64_16410)	28960..29145	+	186	WP_002368099.1	hypothetical protein	-
KKP64_RS16405 (KKP64_16415)	29167..29313	+	147	WP_002368101.1	DpnD/PcfM family protein	-
KKP64_RS16410 (KKP64_16420)	29544..30323	+	780	WP_002368102.1	Cas9 inhibitor AcrIIA9 family protein	-
KKP64_RS16415 (KKP64_16425)	30323..31582	+	1260	WP_002368103.1	PcfJ domain-containing protein	-
KKP64_RS16420 (KKP64_16430)	32232..32960	+	729	WP_002368105.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	ant(6)-Ia / aph(3')-III / erm(B) / cat(pC221)	-	1..75084	75084

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (7-86)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10732.58 Da
• Isoelectric Point: 9.6918

>T203975 WP_002360769.1 NZ_CP075605:27882-28157 [Enterococcus faecalis]
MLEIFYTNQFKKDFKKAKKQGKNLEKLKEVLVLLQEQQTLPPKYKDHALTGNYIGTRECHIEPDWLLIYKIDGDKLILTL
ARIGSHSELFR

Nucleotide

• Length: 276 bp

>T203975 NZ_CP100497:c5132810-5132523 [Klebsiella michiganensis]
ATGCCAATGGAGTTTGAATGGGATGCGAACAAGGCAATAAGTAATCTAAGAAAACATGGAATCCGGTTTGAAGAAGCGGT
GCTCGTCTTTGACGATCCCCGGCATCTTTCCCGACAGGATCGTTATGAGAATGGTGAATATCGCTGGCAAACTATCGGCC
TCGTTCATGGGATCATTGTTATCATGGTTGCGCATTCGGTCCGTTTTGAAAGTGGAACAGAGGTCATTCGTATTATCAGC
GCCCGGAAGTCGGACAGCAAAGAGAGAAATCGTTATGAACATGGTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9772.09 Da
• Isoelectric Point: 4.4791

>AT203975 WP_002369771.1 NZ_CP075605:27623-27889 [Enterococcus faecalis]
VATTKKKPIHVNVDENLKEEAEQLFDDLGLNMTSAITIFLKQSINEQAIPFMINKGNKETLQALKDIKEGNVHGGFSSVE
DLMEDLNA

Nucleotide

• Length: 267 bp

>AT203975 NZ_CP100497:c5132536-5132234 [Klebsiella michiganensis]
ATGAACATGGTTAAACATAAACGTGACAATCCGGATACGCTTACGGCTAAGCGAGACGATGAGCTTAAAGCGCTGGCCAA
CAAAACGGATGATGAAATCGATTATAGCGATATTCCCGCCACAGACGACGCACAGTGGTCAGATGCCGTACGCGGTAAAT
TTTACCGACCGCTAAAAACTCAGGCTTCTGTCCGTATTGATGCCGATATTATGGAGTGGCTTAAACGTCCCGGGAAAGGT
TATCAAACGCGACTGAATGCGATCTTAAGAGAAGCGATGATTCGCGATCTTAACAAGAAATAG

Structures

Toxin

• AlphaFold DB: A0A0M2AEA7

Antitoxin

• AlphaFold DB: A0A0M2AED7