Detailed information of TA system TA203945

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/Peptidase_M78-HTH_19
• Location: 2854932..2855627
• Replicon: chromosome
• Accession: NZ_CP075604
• Organism: Enterococcus faecalis strain 1207/14

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: -
• Locus tag: KKP64_RS14365
• Protein ID: WP_010708389.1
• Coordinates: 2855283..2855627 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: KKP64_RS14360
• Protein ID: WP_016024366.1
• Coordinates: 2854932..2855264 (+)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KKP64_RS14290 (2850726)	2850726..2851103	+	378	WP_002410219.1	hypothetical protein	-
KKP64_RS14295 (2851065)	2851065..2851340	-	276	WP_002382577.1	hypothetical protein	-
KKP64_RS14300 (2851435)	2851435..2851662	-	228	WP_002382575.1	hypothetical protein	-
KKP64_RS14305 (2851662)	2851662..2851985	-	324	WP_002382574.1	hypothetical protein	-
KKP64_RS14310 (2852028)	2852028..2852213	-	186	WP_216788103.1	hypothetical protein	-
KKP64_RS14315 (2852204)	2852204..2852398	-	195	WP_192210295.1	hypothetical protein	-
KKP64_RS14325 (2852706)	2852706..2852879	-	174	WP_010708397.1	hypothetical protein	-
KKP64_RS14330 (2852890)	2852890..2853075	-	186	WP_010708396.1	hypothetical protein	-
KKP64_RS14335 (2853086)	2853086..2853796	-	711	WP_016024367.1	phage regulatory protein	-
KKP64_RS14340 (2853750)	2853750..2853911	-	162	WP_010708394.1	hypothetical protein	-
KKP64_RS14345 (2853922)	2853922..2854098	-	177	WP_010708393.1	helix-turn-helix transcriptional regulator	-
KKP64_RS14350 (2854176)	2854176..2854454	+	279	WP_010708392.1	hypothetical protein	-
KKP64_RS14355 (2854451)	2854451..2854612	-	162	WP_010708391.1	hypothetical protein	-
KKP64_RS14360 (2854932)	2854932..2855264	+	333	WP_016024366.1	helix-turn-helix transcriptional regulator	Antitoxin
KKP64_RS14365 (2855283)	2855283..2855627	+	345	WP_010708389.1	ImmA/IrrE family metallo-endopeptidase	Toxin
KKP64_RS17715 (2855729)	2855729..2856508	+	780	WP_233029440.1	recombinase family protein	-
KKP64_RS17720 (2856521)	2856521..2857096	+	576	WP_233029438.1	zinc ribbon domain-containing protein	-
KKP64_RS14375 (2857118)	2857118..2857693	-	576	WP_025191663.1	DNA repair protein RadC	-
KKP64_RS14380 (2857725)	2857725..2858384	-	660	WP_002385286.1	HAD family hydrolase	-
KKP64_RS14385 (2858368)	2858368..2859690	-	1323	WP_002355117.1	folylpolyglutamate synthase/dihydrofolate synthase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2810453..2862503	52050

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (5-59)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13459.46 Da
• Isoelectric Point: 5.2082

>T203945 WP_010708389.1 NZ_CP075604:2855283-2855627 [Enterococcus faecalis]
MKSIKELVEEYNVELVFTTLHKKACFEPKYGVIFINQDLSTAEQEEAIYHEFKHVKDHADLMALYNIPIFRSKMEAEAEQ
YMLECLIEKNGGQFNYSNVITHYNLKMGQEIYLK

Nucleotide

• Length: 345 bp

>T203945 NZ_CP100486:2926282-2926389 [Escherichia coli]
ATGACGCTCACGCAGTTTGCCATGACTTTCTGGCACGACTTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12589.28 Da
• Isoelectric Point: 5.2520

>AT203945 WP_016024366.1 NZ_CP075604:2854932-2855264 [Enterococcus faecalis]
MTAFDRIKKITDRRGISINELENRVGISQNVLYGWKKKTPGGENLTKVAKYLNISTDYILGIVDNPEPFVEKKTDDLDEV
LDNVMSFDGEPLDDHDREVIRAYLKGRFGK

Nucleotide

• Length: 333 bp

>AT203945 NZ_CP100486:c2926232-2926169 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTATACCTCTCAACGTGCGGGGGTTTTCT