Detailed information of TA system TA203938

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 4560901..4561522
• Replicon: chromosome
• Accession: NZ_CP075595
• Organism: Pseudomonas sp. RC3H12

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: KHO27_RS20830
• Protein ID: WP_102681827.1
• Coordinates: 4561331..4561522 (-)
• Length: 64 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KHO27_RS20825
• Protein ID: WP_214500714.1
• Coordinates: 4560901..4561314 (-)
• Length: 138 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHO27_RS20805 (KHO27_20805)	4556024..4556275	-	252	WP_110697262.1	helix-turn-helix domain-containing protein	-
KHO27_RS20810 (KHO27_20810)	4556364..4558658	-	2295	WP_110697260.1	TonB-dependent siderophore receptor	-
KHO27_RS20815 (KHO27_20815)	4558800..4559519	+	720	WP_214500713.1	response regulator transcription factor	-
KHO27_RS20820 (KHO27_20820)	4559578..4560900	+	1323	WP_214502012.1	ATP-binding protein	-
KHO27_RS20825 (KHO27_20825)	4560901..4561314	-	414	WP_214500714.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
KHO27_RS20830 (KHO27_20830)	4561331..4561522	-	192	WP_102681827.1	type II toxin-antitoxin system HicA family toxin	Toxin
KHO27_RS20835 (KHO27_20835)	4561702..4562247	-	546	WP_102681828.1	HDIG domain-containing protein	-
KHO27_RS20840 (KHO27_20840)	4562339..4563361	-	1023	WP_110697252.1	putative 2-aminoethylphosphonate ABC transporter substrate-binding protein	-
KHO27_RS20845 (KHO27_20845)	4563392..4565116	-	1725	WP_110697250.1	putative 2-aminoethylphosphonate ABC transporter permease subunit	-
KHO27_RS20850 (KHO27_20850)	4565118..4566176	-	1059	WP_110697248.1	putative 2-aminoethylphosphonate ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-58)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 64 a.a.
• Molecular weight: 7033.15 Da
• Isoelectric Point: 10.4613

>T203938 WP_102681827.1 NZ_CP075595:c4561522-4561331 [Pseudomonas sp. RC3H12]
MNSREMIAVIEADGWYLVRSKGSHHHFKHPEKAGLVTIPHPKKDLLPATAASILRQARISYAS

Nucleotide

• Length: 192 bp

>T203938 NZ_CP100486:1279235-1279546 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCTAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 138 a.a.
• Molecular weight: 14931.96 Da
• Isoelectric Point: 4.6025

>AT203938 WP_214500714.1 NZ_CP075595:c4561314-4560901 [Pseudomonas sp. RC3H12]
MLFPIAILPGDDQHAWGVEVPDIPGCFSAGEDLDDAIAMAREAIEGHLELLAQDQQEIPRASKVGVHAANPAYAGCTWAL
IDIDITRYLGKAEKLNITLPAYLLTRIDSYVQNHPEHKSRSGFLAEAALRILQSDGK

Nucleotide

• Length: 414 bp

>AT203938 NZ_CP100486:1279543-1279962 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCCTTACTTCGTACGCTGATTGATCAACATAATTTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
TCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCATATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA