Detailed information of TA system TA203937

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 3976983..3977502
• Replicon: chromosome
• Accession: NZ_CP075595
• Organism: Pseudomonas sp. RC3H12

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A1H9NMR5
• Locus tag: KHO27_RS18015
• Protein ID: WP_023629498.1
• Coordinates: 3977212..3977502 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: KHO27_RS18010
• Protein ID: WP_203648016.1
• Coordinates: 3976983..3977222 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHO27_RS17995 (KHO27_17995)	3972145..3972663	-	519	WP_214500579.1	histidine phosphatase family protein	-
KHO27_RS18000 (KHO27_18000)	3972799..3975102	-	2304	WP_214500580.1	5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase	-
KHO27_RS18005 (KHO27_18005)	3975404..3976891	+	1488	WP_214500581.1	methylenetetrahydrofolate reductase C-terminal domain-containing protein	-
KHO27_RS18010 (KHO27_18010)	3976983..3977222	+	240	WP_203648016.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
KHO27_RS18015 (KHO27_18015)	3977212..3977502	+	291	WP_023629498.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KHO27_RS18020 (KHO27_18020)	3977925..3978521	+	597	WP_214500582.1	hypothetical protein	-
KHO27_RS18025 (KHO27_18025)	3978666..3978896	-	231	WP_102682137.1	hypothetical protein	-
KHO27_RS18030 (KHO27_18030)	3978889..3979029	-	141	WP_214500583.1	hypothetical protein	-
KHO27_RS18035 (KHO27_18035)	3979789..3980478	-	690	WP_250888376.1	AbiV family abortive infection protein	-
KHO27_RS18040 (KHO27_18040)	3981426..3981770	-	345	WP_083567648.1	hypothetical protein	-
KHO27_RS18045 (KHO27_18045)	3981755..3981964	-	210	WP_214500584.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-64)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11378.22 Da
• Isoelectric Point: 10.2588

>T203937 WP_023629498.1 NZ_CP075595:3977212-3977502 [Pseudomonas sp. RC3H12]
MTYNLDFDARALKEWQKLGDTIRQQLKKKLATVLLNPRVEANRLHGFPDCYKIKLRSSGYRLVYQVIDHQIVVFVVAVDK
RERDQVYRKAAERLTE

Nucleotide

• Length: 291 bp

>T203937 NZ_CP100486:1204634-1204969 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAGCTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9034.45 Da
• Isoelectric Point: 4.8146

>AT203937 WP_203648016.1 NZ_CP075595:3976983-3977222 [Pseudomonas sp. RC3H12]
MASINVRIDDDLKVRAYRELEKLGVTPSELMRQALQYVAERGQLPFKPVLLTEEDEALLATVRERLAAPQRVRVTLDDL

Nucleotide

• Length: 240 bp

>AT203937 NZ_CP100486:1204386-1204634 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A1H9NMR5