Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5301621..5302246 Replicon chromosome
Accession NZ_CP075591
Organism Klebsiella pneumoniae strain ST17

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KJE29_RS26085 Protein ID WP_002882817.1
Coordinates 5301621..5302004 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KJE29_RS26090 Protein ID WP_004150355.1
Coordinates 5302004..5302246 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KJE29_RS26070 (5298987) 5298987..5299889 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KJE29_RS26075 (5299886) 5299886..5300521 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KJE29_RS26080 (5300518) 5300518..5301447 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KJE29_RS26085 (5301621) 5301621..5302004 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KJE29_RS26090 (5302004) 5302004..5302246 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KJE29_RS26095 (5302451) 5302451..5303368 + 918 WP_023302328.1 alpha/beta hydrolase -
KJE29_RS26100 (5303382) 5303382..5304323 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KJE29_RS26105 (5304368) 5304368..5304805 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KJE29_RS26110 (5304802) 5304802..5305662 - 861 WP_004146232.1 virulence factor BrkB family protein -
KJE29_RS26115 (5305656) 5305656..5306255 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T203933 WP_002882817.1 NZ_CP075591:c5302004-5301621 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T203933 NZ_CP100486:c665325-664861 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTACAGCGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAGCCCATTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT203933 WP_004150355.1 NZ_CP075591:c5302246-5302004 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT203933 NZ_CP100486:c665660-665325 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACACTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References