Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4741804..4742614 Replicon chromosome
Accession NZ_CP075591
Organism Klebsiella pneumoniae strain ST17

Toxin (Protein)


Gene name KacT Uniprot ID A0A332NV98
Locus tag KJE29_RS23450 Protein ID WP_014908042.1
Coordinates 4741804..4742337 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID -
Locus tag KJE29_RS23455 Protein ID WP_023343051.1
Coordinates 4742348..4742614 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KJE29_RS23445 (4740635) 4740635..4741756 + 1122 WP_012737335.1 cupin domain-containing protein -
KJE29_RS23450 (4741804) 4741804..4742337 - 534 WP_014908042.1 type II toxin-antitoxin system toxin KacT Toxin
KJE29_RS23455 (4742348) 4742348..4742614 - 267 WP_023343051.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KJE29_RS23460 (4742717) 4742717..4744150 - 1434 WP_023343050.1 Cu(+)/Ag(+) sensor histidine kinase -
KJE29_RS23465 (4744140) 4744140..4744823 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
KJE29_RS23470 (4744996) 4744996..4746381 + 1386 WP_024623207.1 efflux transporter outer membrane subunit -
KJE29_RS23475 (4746399) 4746399..4746742 + 344 Protein_4599 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19795.68 Da        Isoelectric Point: 5.2614

>T203930 WP_014908042.1 NZ_CP075591:c4742337-4741804 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGLGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T203930 NZ_CP100486:250792-250899 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGCATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10021.46 Da        Isoelectric Point: 10.5807

>AT203930 WP_023343051.1 NZ_CP075591:c4742614-4742348 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRRVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT203930 NZ_CP100486:c250734-250680 [Escherichia coli]
CAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A332NV98

Antitoxin

Source ID Structure

References