Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4131959..4132578 Replicon chromosome
Accession NZ_CP075591
Organism Klebsiella pneumoniae strain ST17

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KJE29_RS20490 Protein ID WP_002892050.1
Coordinates 4132360..4132578 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KJE29_RS20485 Protein ID WP_002892066.1
Coordinates 4131959..4132333 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KJE29_RS20475 (4127111) 4127111..4128304 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KJE29_RS20480 (4128327) 4128327..4131473 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
KJE29_RS20485 (4131959) 4131959..4132333 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KJE29_RS20490 (4132360) 4132360..4132578 + 219 WP_002892050.1 HHA domain-containing protein Toxin
KJE29_RS20495 (4132737) 4132737..4133303 + 567 WP_002892030.1 maltose O-acetyltransferase -
KJE29_RS20500 (4133275) 4133275..4133415 - 141 WP_004147370.1 hypothetical protein -
KJE29_RS20505 (4133436) 4133436..4133906 + 471 WP_002892026.1 YlaC family protein -
KJE29_RS20510 (4133881) 4133881..4135332 - 1452 WP_023302231.1 PLP-dependent aminotransferase family protein -
KJE29_RS20515 (4135433) 4135433..4136131 + 699 WP_002892021.1 GNAT family protein -
KJE29_RS20520 (4136128) 4136128..4136268 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KJE29_RS20525 (4136268) 4136268..4136531 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T203929 WP_002892050.1 NZ_CP075591:4132360-4132578 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T203929 NZ_CP100486:195107-195292 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT203929 WP_002892066.1 NZ_CP075591:4131959-4132333 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT203929 NZ_CP100486:195309-195719 [Escherichia coli]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACAACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGA
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGTGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCGAAAAGGTTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTACGCCAACAGAAGTGCGTTTCTGGCAGAAGCTGCCCGGCGTGTATTGC
CGGGCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References