Detailed information of TA system TA203916

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 5917477..5919203
• Replicon: chromosome
• Accession: NZ_CP075567
• Organism: Pseudomonas fitomaticsae strain FIT81

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: A0A2N8H983
• Locus tag: KJY40_RS26820
• Protein ID: WP_074691609.1
• Coordinates: 5917477..5918121 (+)
• Length: 215 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: KJY40_RS26825
• Protein ID: WP_085747631.1
• Coordinates: 5918118..5919203 (+)
• Length: 362 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KJY40_RS26795 (KJY40_26735)	5913293..5913820	+	528	WP_007933577.1	inorganic diphosphatase	-
KJY40_RS26800 (KJY40_26740)	5914042..5914779	+	738	WP_230733750.1	helix-turn-helix transcriptional regulator	-
KJY40_RS26805 (KJY40_26745)	5915232..5915663	+	432	WP_230733751.1	hypothetical protein	-
KJY40_RS26810 (KJY40_26750)	5915794..5916714	+	921	WP_230733754.1	restriction endonuclease	-
KJY40_RS26815 (KJY40_26755)	5916899..5917480	+	582	WP_230733756.1	DarT ssDNA thymidine ADP-ribosyltransferase family protein	-
KJY40_RS26820 (KJY40_26760)	5917477..5918121	+	645	WP_074691609.1	DUF4433 domain-containing protein	Toxin
KJY40_RS26825 (KJY40_26765)	5918118..5919203	+	1086	WP_085747631.1	macro domain-containing protein	Antitoxin
KJY40_RS26830 (KJY40_26770)	5919391..5919951	+	561	WP_085747630.1	hypothetical protein	-
KJY40_RS26835 (KJY40_26775)	5919967..5920401	+	435	WP_039772002.1	hypothetical protein	-
KJY40_RS26840 (KJY40_26780)	5920425..5921237	-	813	WP_230733758.1	DUF2135 domain-containing protein	-
KJY40_RS26845 (KJY40_26785)	5921241..5922860	-	1620	WP_230733759.1	DUF2300 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (14-213)

Antitoxin

• Macro domain (18-130)

Sequences

Toxin

Protein

• Length: 215 a.a.
• Molecular weight: 24914.33 Da
• Isoelectric Point: 6.5693

>T203916 WP_074691609.1 NZ_CP075567:5917477-5918121 [Pseudomonas fitomaticsae]
MNYTSLNPEKALIWRIVHRDNLPWILDNGLHCANSEVQAPHYVNIGNVDLIDKRRSRQVPIAPEGVLADYVPFYFTPFSV
MMQNIHSGWSVQQRSNDEIVILVSSLHRVEELGLPFVFTNAHAYPSWTDYYSDLANLDQIDWTILQRRDFKRDPDDPRKI
ERYQAEALIHHHLPITGLLGIMCHTDAMKERIEQDVAARGLTLSVHARPKWYFQ

Nucleotide

• Length: 645 bp

>T203916 NZ_CP100478:c4640758-4640474 [Klebsiella pneumoniae]
ATGACCTATGAACTGGCGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 362 a.a.
• Molecular weight: 40769.94 Da
• Isoelectric Point: 6.5099

>AT203916 WP_085747631.1 NZ_CP075567:5918118-5919203 [Pseudomonas fitomaticsae]
MIRFTQGNLLEAKTEALVNTVNTVGVMGKGIALMFKERFAQNYRLYAAACKAKEVETGKMHVTATNELEGPRWIVNFPTK
RHWRSPSQMAWITEGLHDLRRFLIENDVKSVAIPPLGAGNGGLKWPEVREQIVEVLGDLDVDVLVFEPTNQYLNVAKRNG
VETLTPARALIAELVRRYWVLGMECSLLEIQKLAWFLERAIEKLPSTENPLNLKFIAHKFGPYANRLEHLLNNLDGSYLH
CDKRISDAEITDVIWFDQDRKAFLQTYLKTEAREYSQALERTAELIDGFESPFGMELLATVDWLLIKEGVVPTVSAVREA
LRNWDGGGGAAVRKNKLFDDQALEIAINRLISSSFSPKIAA

Nucleotide

• Length: 1086 bp

>AT203916 NZ_CP100478:c4640990-4640748 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A2N8H983