Detailed information of TA system TA203912

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2051080..2052119
• Replicon: chromosome
• Accession: NZ_CP075567
• Organism: Pseudomonas fitomaticsae strain FIT81

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: KJY40_RS09275
• Protein ID: WP_230736323.1
• Coordinates: 2051080..2051655 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: KJY40_RS09280
• Protein ID: WP_230736325.1
• Coordinates: 2051652..2052119 (-)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KJY40_RS09250 (KJY40_09235)	2046407..2048395	+	1989	WP_230736317.1	heme lyase CcmF/NrfE family subunit	-
KJY40_RS09255 (KJY40_09240)	2048392..2048928	+	537	WP_103385710.1	DsbE family thiol:disulfide interchange protein	-
KJY40_RS09260 (KJY40_09245)	2048925..2049395	+	471	WP_011333089.1	cytochrome c-type biogenesis protein CcmH	-
KJY40_RS09265 (KJY40_09250)	2049392..2050594	+	1203	WP_230736319.1	c-type cytochrome biogenesis protein CcmI	-
KJY40_RS09270 (KJY40_09255)	2050620..2051027	+	408	WP_230736321.1	hypothetical protein	-
KJY40_RS09275 (KJY40_09260)	2051080..2051655	-	576	WP_230736323.1	PIN domain-containing protein	Toxin
KJY40_RS09280 (KJY40_09265)	2051652..2052119	-	468	WP_230736325.1	helix-turn-helix domain-containing protein	Antitoxin
KJY40_RS09285	2052204..2052296	-	93	Protein_1835	LysR family transcriptional regulator	-
KJY40_RS09290 (KJY40_09270)	2052399..2052803	+	405	Protein_1836	type II toxin-antitoxin system HicB family antitoxin	-
KJY40_RS09295 (KJY40_09275)	2052850..2054037	-	1188	WP_230736327.1	MFS transporter	-
KJY40_RS09300 (KJY40_09280)	2054279..2054899	+	621	WP_039769760.1	DUF5666 domain-containing protein	-
KJY40_RS09305 (KJY40_09285)	2054955..2055953	-	999	WP_127797317.1	sulfate ABC transporter substrate-binding protein	-
KJY40_RS09310 (KJY40_09290)	2056060..2056884	-	825	WP_230736329.1	ion transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-115)

Antitoxin

• SinI-like, DNA-binding domain (84-130)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21559.87 Da
• Isoelectric Point: 5.9178

>T203912 WP_230736323.1 NZ_CP075567:c2051655-2051080 [Pseudomonas fitomaticsae]
VRHSSFTALYDACVLYPAPLRDFLMWLALSGRFRARWTQEIHCEWKRNLLKNRPDLTMEQLDRTSGLMDQAIPDACVHDY
EDLIAGLTLPDCDDRHVLAAAIRCGASVIVTFNQKDFPCKALEPFGIEAQHPDEFVDNLLGLDPAAVVAAAQKQRRQLKM
PAMDVEPFLDLLQRQGLIKSVQALSSYHAIL

Nucleotide

• Length: 576 bp

>T203912 NZ_CP100478:1890375-1890477 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17300.75 Da
• Isoelectric Point: 6.4962

>AT203912 WP_230736325.1 NZ_CP075567:c2052119-2051652 [Pseudomonas fitomaticsae]
MSTVIHPSISLPVEREVKAAIEGQRALAAYLATHFETQHIQIFDEQNEAHRVELPTSALRLLIDILAELAAGNAVQVVPV
HAELTTQEAADLLNVSRPHLIKLLESGELSYHKTGKHRRVRFADLMDYKTRRTTVSEQAMALLAEQAQALRTGYE

Nucleotide

• Length: 468 bp

>AT203912 NZ_CP100478:c1890484-1890339 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT