Detailed information of TA system TA203906

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 782674..783297
• Replicon: chromosome
• Accession: NZ_CP075566
• Organism: Pseudomonas fluorescens strain G20-18

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0K1QNY8
• Locus tag: KJF94_RS03605
• Protein ID: WP_017336896.1
• Coordinates: 783118..783297 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KJF94_RS03600
• Protein ID: WP_214381246.1
• Coordinates: 782674..783081 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KJF94_RS03570 (KJF94_03570)	778167..778577	-	411	WP_214381238.1	low affinity iron permease family protein	-
KJF94_RS03575 (KJF94_03575)	778683..779222	-	540	WP_214384708.1	AidA/PixA family protein	-
KJF94_RS03580 (KJF94_03580)	779349..779852	-	504	WP_017336890.1	inclusion body family protein	-
KJF94_RS03585 (KJF94_03585)	780245..781015	+	771	WP_214381240.1	trans-aconitate 2-methyltransferase	-
KJF94_RS03590 (KJF94_03590)	781441..781710	-	270	WP_214381242.1	short-chain dehydrogenase	-
KJF94_RS03595 (KJF94_03595)	782179..782490	-	312	WP_214381244.1	hypothetical protein	-
KJF94_RS03600 (KJF94_03600)	782674..783081	-	408	WP_214381246.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
KJF94_RS03605 (KJF94_03605)	783118..783297	-	180	WP_017336896.1	type II toxin-antitoxin system HicA family toxin	Toxin
KJF94_RS03615 (KJF94_03615)	783851..784735	-	885	WP_214381248.1	alpha/beta hydrolase	-
KJF94_RS03620 (KJF94_03620)	785050..785742	-	693	WP_214381250.1	pseudouridine synthase	-
KJF94_RS03625 (KJF94_03625)	785800..785994	-	195	WP_084322636.1	cysteine-rich CWC family protein	-
KJF94_RS03630 (KJF94_03630)	786001..787488	-	1488	WP_214381252.1	sensor domain-containing diguanylate cyclase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6676.95 Da
• Isoelectric Point: 11.1923

>T203906 WP_017336896.1 NZ_CP075566:c783297-783118 [Pseudomonas fluorescens]
VNSRFLISQIVADGWYLVRIKGSHHHFKHPTKPGLVTVPHPKKDLLKKTALSILQQALL

Nucleotide

• Length: 180 bp

>T203906 NZ_CP100476:2779823-2780260 [Mesorhizobium sp. C120A]
GTGACCTTCCTGCTCGACGTCAATGTGTTGATTGCGTTGATCGATCCGGCTCACGTCGCGCATGAAGATGCCCATCGGTG
GTTCCAGTCCACCGGGCATTTATCATGGGCAACATGTCCCATTACCGAGAATGGCGTCATCAGGATTGTAAGCAACCCGA
AATACCCCAATTCTCCGGGTTCGCCCGCCGTGGTGGCGCAGATTGTCGGCAAGCTGCATGCCCTTTCAGGACATCAGTTC
TGGCCTGACGAGATCAGCCTTGTCGGTTCCAACGACATCGATGCAGCCAGGATCCTCACCCCGGCACAGGTAACGGATAG
CTATCTGTTGGGACTCGCCAAGGCGCGTGACGGCCAATTGGCGACGTTTGACCGAAAGCTGTCGACGGCAGCGGTAAAGG
GAGGGAGGTCGATCCTCCACCTGATCCCAAGCGAATGA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14671.65 Da
• Isoelectric Point: 4.4988

>AT203906 WP_214381246.1 NZ_CP075566:c783081-782674 [Pseudomonas fluorescens]
MLYPIAISMGDNEHAWGVEVPDIPGCFSAGEDLDDAMAMAREAIEGHFEILAEDGSPIPPANKVTLHAANPHYAGCTWAV
VDIDVTKYLGKAQKLNITLPGYLLNRIDEYVLHHPEEKSRSGFLASAALKVLQES

Nucleotide

• Length: 408 bp

>AT203906 NZ_CP100476:2779599-2779826 [Mesorhizobium sp. C120A]
GTGAGAACCACACTTGCAATCGATGACGATGTCCTACTTGCGGCCAAGGCCATGGCGCGACAGCAGGATCGCAGCGTTGG
CGACGTCATTTCCGATCTGGCTCGCCGCTCACTGCGCAGGCCCCCGACCGGCGGTGAACGCAACGGCATCCCCTTGCTTG
CATCGCGGCCCGACAGCCCGATGGTCGATCTCGAAATGGTCAATGCGCTTCGCGACGAATTGCCGTGA

Structures

Toxin

• AlphaFold DB: A0A0K1QNY8