Detailed information of TA system TA203905

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 773252..773765
• Replicon: chromosome
• Accession: NZ_CP075566
• Organism: Pseudomonas fluorescens strain G20-18

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: KJF94_RS03535
• Protein ID: WP_214381228.1
• Coordinates: 773252..773536 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: KJF94_RS03540
• Protein ID: WP_214381229.1
• Coordinates: 773526..773765 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KJF94_RS03515 (KJF94_03515)	768369..769277	+	909	WP_214381223.1	LysR family transcriptional regulator	-
KJF94_RS03520 (KJF94_03520)	769508..770701	+	1194	WP_214381225.1	CaiB/BaiF CoA-transferase family protein	-
KJF94_RS03525 (KJF94_03525)	770698..771624	+	927	WP_214381226.1	hydroxymethylglutaryl-CoA lyase	-
KJF94_RS03530 (KJF94_03530)	771803..773134	+	1332	WP_214381227.1	MFS transporter	-
KJF94_RS03535 (KJF94_03535)	773252..773536	-	285	WP_214381228.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KJF94_RS03540 (KJF94_03540)	773526..773765	-	240	WP_214381229.1	ribbon-helix-helix protein, CopG family	Antitoxin
KJF94_RS03545 (KJF94_03545)	773927..774661	+	735	WP_214381230.1	hypothetical protein	-
KJF94_RS03550 (KJF94_03550)	774692..775156	+	465	WP_214381231.1	fosfomycin resistance glutathione transferase	-
KJF94_RS03555 (KJF94_03555)	775175..775812	+	638	Protein_702	LysE family translocator	-
KJF94_RS03560 (KJF94_03560)	775900..777249	+	1350	WP_214381233.1	diguanylate cyclase	-
KJF94_RS30035	777366..777524	+	159	WP_250548216.1	stress protein	-
KJF94_RS03565 (KJF94_03565)	777598..778101	+	504	WP_214381236.1	DUF4142 domain-containing protein	-
KJF94_RS03570 (KJF94_03570)	778167..778577	-	411	WP_214381238.1	low affinity iron permease family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-89)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-41)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10891.53 Da
• Isoelectric Point: 10.5255

>T203905 WP_214381228.1 NZ_CP075566:c773536-773252 [Pseudomonas fluorescens]
MRVEWLRTASKNLDDEAAYIAMENPKAAADFVQAILASVDHLAHFPATGREGRLPGTREWVLPDRPYLIPYRVRQGRLQV
LRVFHTRRLPPTNW

Nucleotide

• Length: 285 bp

>T203905 NZ_CP100476:2751556-2751954 [Mesorhizobium sp. C120A]
ATGCGGTTCATGCTGGACACCAACATTCTCAGCGACATGATCCGAAACCCTGCCGGCAAGGCTGCCAGTACGGCGGTTCG
CGCGGGAGACGGTGCCCTGTGCACCAGCATCGTTGTCGCCTCGGAGCTGCGTTATGGCTGCGCCCGCAAGGGATCGGCCA
AGCTGCTCGCCAAGGTCGAGCAATTGCTCGCCGAGTTACCTGTGCTGCCGCTCGACGTGCCGATGGATAACGAATACGGA
GCACTTCGTGCCGAGCTGGAGGCCAGAGGCCAGCCAATCGGCCACAATGACCTTTTCATTGCTGCCCACGTCTGCGCGCT
GGGGACGACGCTGGTTACGGCCAACACCGGTGAGTTCAGCCGGATCAAGGGCCTGAAGGTCGAGAACTGGCTGGAGTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8734.94 Da
• Isoelectric Point: 4.4013

>AT203905 WP_214381229.1 NZ_CP075566:c773765-773526 [Pseudomonas fluorescens]
MSVMSLRLPDEIADTLATLSKATGRSKSFLAVDALREYLAREAWQIEEIQKALIEADAGDFVTPEEVNAIANKWIANAR

Nucleotide

• Length: 240 bp

>AT203905 NZ_CP100476:2751284-2751556 [Mesorhizobium sp. C120A]
ATGCCGCAGCACCGTCACTCAATACCACCGCCCAGGGAGGCGAAGCTCTTCCGCAACAACCGCAGCCAGGCGGTGCGCAT
TCCCGTCGAGTTCGAGCTTCCCGGCGAAAAAGTCCTCATCAGCCGCGACGGCGATCGCCTGGTTATCGAGCCAGTCCGTA
AACCAGGCCTCACCGCCTTGCTCACCCAATGGGCGAAAGAGCCGCCGCTTGGTCCTGAAGACGACTTTCCCGAGATCCAT
GATGCGCCCGTGGGGCCTGAGGATATCTTTTGA