Detailed information of TA system TA203895

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1140000..1140529
• Replicon: chromosome
• Accession: NZ_CP075563
• Organism: Staphylococcus aureus strain Sta2021027

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KJP35_RS05505
• Protein ID: WP_000621175.1
• Coordinates: 1140167..1140529 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: KJP35_RS05500
• Protein ID: WP_000948331.1
• Coordinates: 1140000..1140170 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KJP35_RS05470 (1135036)	1135036..1135596	+	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-
KJP35_RS05475 (1135805)	1135805..1136284	+	480	WP_001287079.1	hypothetical protein	-
KJP35_RS05480 (1136277)	1136277..1137860	+	1584	WP_045177766.1	PH domain-containing protein	-
KJP35_RS05485 (1137847)	1137847..1138338	+	492	WP_072353916.1	PH domain-containing protein	-
KJP35_RS05490 (1138342)	1138342..1138701	+	360	WP_000581197.1	holo-ACP synthase	-
KJP35_RS05495 (1138767)	1138767..1139915	+	1149	WP_001281154.1	alanine racemase	-
KJP35_RS05500 (1140000)	1140000..1140170	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
KJP35_RS05505 (1140167)	1140167..1140529	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KJP35_RS05510 (1140879)	1140879..1141880	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
KJP35_RS05515 (1141999)	1141999..1142325	+	327	WP_001052491.1	anti-sigma factor antagonist	-
KJP35_RS05520 (1142327)	1142327..1142806	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
KJP35_RS05525 (1142781)	1142781..1143551	+	771	WP_001041102.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T203895 WP_000621175.1 NZ_CP075563:1140167-1140529 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T203895 NZ_CP100475:3590886-3591323 [Mesorhizobium sp. C372A]
GTGACCTTCCTGCTCGACGTCAATGTCTTGATTGCGTTGATCGATCCGGCTCACGTCGCGCATGAAGATGCCCATCGGTG
GTTTCAATCCACCGGGCATTTATCATGGGCAACATGTCCCATTACCGAGAATGGCGTCATCAGGATTGTAAGCAACCCGA
AATACCCCAATTCTCCGGGTTCGCCGGCCGTGGTGGCGCAGATTGTCGGCAAGCTGCATGCCCTTTCAGGACATCAGTTC
TGGCCTGACGAGATCAGCCTTGTCGGTTCCAACGACATCGATGCAGCCAGGATCCTCACCCCGGCACAGGTAACGGATAG
TTATCTGTTGGGACTCGCCAAGGCGCGTGACGGCCAATTGGCGACGTTTGACCGAAAGCTGTCGACGGCAGCGGTAAAGG
GAGGGAGGTCGATCCTCCACCTGATCCCAAGCGAATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT203895 WP_000948331.1 NZ_CP075563:1140000-1140170 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT203895 NZ_CP100475:3590662-3590889 [Mesorhizobium sp. C372A]
GTGAGAACCACACTTGCAATCGATGACGATGTCCTACTTGCGGCCAAGGCCATGGCGCGACAGCAGGATCGCAGCGTTGG
CGATGTCATTTCCGATTTGGCTCGCCGCTCACTGCGCCGGCCACCGACCGGCGGTGAACGCAACGGCATCCCCTTGCTTG
CATCGCGGCCCGACGGCCCGATGGTCGATCTCGAAATGGTCAATGCGCTTCGCGACGAATTGCCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1