Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 81493..83116 Replicon plasmid pRGF99-1-tmexCD
Accession NZ_CP075559
Organism Klebsiella pneumoniae strain RGF99-1

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag KI681_RS27265 Protein ID WP_000088044.1
Coordinates 81791..83116 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag KI681_RS27260 Protein ID WP_012477377.1
Coordinates 81493..81786 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KI681_RS27230 (KI681_27235) 76701..77066 - 366 WP_000995360.1 mercury resistance co-regulator MerD -
KI681_RS27235 (KI681_27240) 77084..78769 - 1686 WP_003156770.1 mercury(II) reductase -
KI681_RS27240 (KI681_27245) 78841..79116 - 276 WP_003131987.1 mercury resistance system periplasmic binding protein MerP -
KI681_RS27245 (KI681_27250) 79129..79479 - 351 WP_003131974.1 mercuric ion transporter MerT -
KI681_RS27250 (KI681_27255) 79551..79985 + 435 WP_003131969.1 mercury resistance transcriptional regulator MerR -
KI681_RS27255 (KI681_27260) 80064..81068 + 1005 WP_000427623.1 IS110-like element IS4321 family transposase -
KI681_RS27260 (KI681_27265) 81493..81786 + 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
KI681_RS27265 (KI681_27270) 81791..83116 + 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
KI681_RS27270 (KI681_27275) 83177..83383 + 207 WP_000134171.1 hypothetical protein -
KI681_RS27275 (KI681_27280) 83484..83894 + 411 WP_000985909.1 hypothetical protein -
KI681_RS27280 (KI681_27285) 83907..84722 + 816 WP_001641519.1 HNH endonuclease signature motif containing protein -
KI681_RS27285 (KI681_27290) 84975..85400 + 426 WP_000278471.1 hypothetical protein -
KI681_RS27290 (KI681_27295) 85949..86257 + 309 WP_001224686.1 hypothetical protein -
KI681_RS27295 (KI681_27300) 86273..87130 + 858 WP_000784387.1 HNH endonuclease signature motif containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(6)-Id / msr(E) / mph(E) / aph(3')-Ia / aph(3'')-Ib / ant(2'')-Ia / blaTEM-1B / aac(3)-IId - 1..317811 317811
- flank IS/Tn aph(3'')-Ib / aph(6)-Id - 74736..81068 6332


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T203867 WP_000088044.1 NZ_CP075559:81791-83116 [Klebsiella pneumoniae]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T203867 NZ_CP100429:c2432778-2432611 [Fusobacterium polymorphum]
ATGTATAAAATTAAAACTACAACTAAATTTGAAAAAGACTTAAAATTATTAAAAGAAGTCATTGAGATATTATCTAAAGG
AGAAGAATATCAAGATCATTATTTACAGGGAAATTACTCTGGCTTTAAAGAATATCATATAAAACCTGATTGGTTACTGG
AGTTATAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT203867 WP_012477377.1 NZ_CP075559:81493-81786 [Klebsiella pneumoniae]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp

>AT203867 NZ_CP100429:c2432977-2432780 [Fusobacterium polymorphum]
TTGGGGCTTAATATATCAGTTACAATAAATTTATTTCTAAAAAAATGTATAAATGAAAAAGGAATACCATTTGACTTAAA
GATTCCAAATAAAGAAACAATAGAAACAATGGAAGAAACAAATAAAATATTAAATGGAGATATAGAGAGAAAATCATATA
AAAATGTTGACGAGTTATTTGAAGATTTAGGCATATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References