Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5872..6397 Replicon plasmid pRGF99-1-75k
Accession NZ_CP075554
Organism Klebsiella pneumoniae strain RGF99-1

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag KI681_RS26240 Protein ID WP_013023785.1
Coordinates 5872..6177 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag KI681_RS26245 Protein ID WP_001568025.1
Coordinates 6179..6397 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KI681_RS26210 (KI681_26210) 1583..2209 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
KI681_RS28695 2206..2508 + 303 WP_004197636.1 hypothetical protein -
KI681_RS26220 (KI681_26220) 2948..3742 - 795 WP_004197635.1 site-specific integrase -
KI681_RS26225 (KI681_26225) 3940..4956 - 1017 WP_017899884.1 hypothetical protein -
KI681_RS26230 (KI681_26230) 4967..5281 - 315 WP_053389906.1 hypothetical protein -
KI681_RS26235 (KI681_26235) 5308..5703 - 396 WP_017899885.1 hypothetical protein -
KI681_RS26240 (KI681_26240) 5872..6177 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
KI681_RS26245 (KI681_26245) 6179..6397 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
KI681_RS26250 (KI681_26255) 7215..7505 - 291 WP_013023783.1 hypothetical protein -
KI681_RS26255 (KI681_26260) 7502..8629 - 1128 WP_013023782.1 DUF4238 domain-containing protein -
KI681_RS26260 (KI681_26265) 8663..10255 - 1593 WP_015344964.1 hypothetical protein -
KI681_RS26265 (KI681_26270) 10462..11241 - 780 WP_013023780.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(3')-Ia / aac(3)-IId / aac(6')-Ib-cr / ARR-3 / dfrA27 / aadA16 / qacE / sul1 / mph(A) / tet(A) / floR / blaTEM-1B / blaCTX-M-3 / qnrS1 - 1..75824 75824


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T203866 WP_013023785.1 NZ_CP075554:c6177-5872 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T203866 NZ_CP100429:c2352955-2352695 [Fusobacterium polymorphum]
ATGAATAATTTGGTATGGACACATAAAGCTTGGCAAGATTACCTATATTGGCAAACTCAGGATAAAAAAACTTTAAAAAA
GATAAATGAATTAGTCAAAGATATTGGAAGAAATGGAGCTTTAAATGGGATAGGCAAGCCAGAAGTATTAAAAAATGAAA
ATGCATATAGTAGAAGAATTGATGAAAAAAATAGACTGGTTTATAGAATTGTAGATGGTTTTATTTGGATAATTGCTTGT
AAAGGGCATTATGAGGAATAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT203866 WP_001568025.1 NZ_CP075554:c6397-6179 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT203866 NZ_CP100429:c2353202-2352948 [Fusobacterium polymorphum]
ATGTTAGCAATTAATTATACAACTTTGAGAACCAATTTAAAAAGCTATTTTGATAAAGCAGTAGACAATGATGAAGATAT
TATTATAACAAGAAAAAATGAAAGAAATGTTGTTCTATTAAGTCTTGATAAATACAATGAGTTTTTAAAAGCTATGAGAA
ATCTTGAATATATGACAAAAATAAGAGAAGGGATAGCTGAATTAGAGGCAGGAAAAGGAGAAATTCATGATTTAATAGAG
GTTGATGATGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References