Detailed information of TA system TA203865

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 99703..100439
• Replicon: plasmid pRGF99-1-214k
• Accession: NZ_CP075553
• Organism: Klebsiella pneumoniae strain RGF99-1

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A8T5ZF70
• Locus tag: KI681_RS25580
• Protein ID: WP_004187044.1
• Coordinates: 99957..100439 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: KI681_RS25575
• Protein ID: WP_003026799.1
• Coordinates: 99703..99969 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KI681_RS25560 (KI681_25560)	95175..96569	+	1395	WP_032439652.1	cytosine permease	-
KI681_RS25565 (KI681_25565)	96581..97789	+	1209	WP_032448288.1	imidazolonepropionase	-
KI681_RS25570 (KI681_25570)	97782..98591	+	810	WP_023329017.1	N-formylglutamate deformylase	-
KI681_RS25575 (KI681_25575)	99703..99969	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
KI681_RS25580 (KI681_25580)	99957..100439	+	483	WP_004187044.1	GNAT family N-acetyltransferase	Toxin
KI681_RS25585 (KI681_25585)	100650..101996	+	1347	WP_077251107.1	ISNCY family transposase	-
KI681_RS25590 (KI681_25590)	102156..102860	+	705	WP_031591821.1	toll/interleukin-1 receptor domain-containing protein	-
KI681_RS25595 (KI681_25595)	103137..104483	+	1347	WP_077254728.1	ISNCY family transposase	-
KI681_RS25600 (KI681_25600)	104532..104930	+	399	WP_032422684.1	helix-turn-helix domain-containing protein	-
KI681_RS25605 (KI681_25605)	105078..105188	-	111	Protein_104	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	iutA / iucD / iucC / iucB / iucA	1..214082	214082

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17280.93 Da
• Isoelectric Point: 8.7197

>T203865 WP_004187044.1 NZ_CP075553:99957-100439 [Klebsiella pneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTYERTLFLKLP

Nucleotide

• Length: 483 bp

>T203865 NZ_CP100428:2803499-2803651 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATTTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT203865 WP_003026799.1 NZ_CP075553:99703-99969 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT203865 NZ_CP100428:2802875-2803474 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGACACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66