Detailed information of TA system TA203864

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 54795..55438
• Replicon: plasmid pRGF99-1-214k
• Accession: NZ_CP075553
• Organism: Klebsiella pneumoniae strain RGF99-1

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: KI681_RS25355
• Protein ID: WP_016236302.1
• Coordinates: 55022..55438 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: KI681_RS25350
• Protein ID: WP_001261282.1
• Coordinates: 54795..55025 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KI681_RS25320 (KI681_25315)	49804..50979	+	1176	Protein_48	IS3 family transposase	-
KI681_RS25325 (KI681_25325)	51361..52146	-	786	WP_050485696.1	site-specific integrase	-
KI681_RS25330 (KI681_25330)	52192..52713	-	522	WP_032448294.1	hypothetical protein	-
KI681_RS25335 (KI681_25335)	52771..53163	-	393	WP_074422814.1	hypothetical protein	-
KI681_RS25340 (KI681_25340)	53272..54216	-	945	WP_032448293.1	hypothetical protein	-
KI681_RS25345 (KI681_25345)	54416..54838	-	423	WP_050485703.1	hypothetical protein	-
KI681_RS25350 (KI681_25350)	54795..55025	+	231	WP_001261282.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KI681_RS25355 (KI681_25355)	55022..55438	+	417	WP_016236302.1	type II toxin-antitoxin system VapC family toxin	Toxin
KI681_RS25360 (KI681_25360)	55512..57074	+	1563	WP_004206609.1	AAA family ATPase	-
KI681_RS25365 (KI681_25365)	57059..58081	+	1023	WP_032439672.1	helicase UvrD	-
KI681_RS25370 (KI681_25370)	58626..59534	+	909	WP_137877090.1	HNH endonuclease	-
KI681_RS25375 (KI681_25375)	59720..60070	-	351	WP_004187110.1	DUF305 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	iutA / iucD / iucC / iucB / iucA	1..214082	214082

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15091.55 Da
• Isoelectric Point: 7.1084

>T203864 WP_016236302.1 NZ_CP075553:55022-55438 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLELAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T203864 NZ_CP100428:2635671-2636033 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT203864 WP_001261282.1 NZ_CP075553:54795-55025 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT203864 NZ_CP100428:2635504-2635674 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GZG3