Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5127760..5128385 Replicon chromosome
Accession NZ_CP075552
Organism Klebsiella pneumoniae strain RGF99-1

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KI681_RS24735 Protein ID WP_002882817.1
Coordinates 5127760..5128143 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KI681_RS24740 Protein ID WP_004150355.1
Coordinates 5128143..5128385 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KI681_RS24720 (KI681_24715) 5125126..5126028 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KI681_RS24725 (KI681_24720) 5126025..5126660 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KI681_RS24730 (KI681_24725) 5126657..5127586 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KI681_RS24735 (KI681_24730) 5127760..5128143 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KI681_RS24740 (KI681_24735) 5128143..5128385 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KI681_RS24745 (KI681_24740) 5128590..5129507 + 918 WP_004178029.1 alpha/beta hydrolase -
KI681_RS24750 (KI681_24745) 5129521..5130462 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KI681_RS24755 (KI681_24750) 5130507..5130944 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KI681_RS24760 (KI681_24755) 5130941..5131801 - 861 WP_004146232.1 virulence factor BrkB family protein -
KI681_RS24765 (KI681_24760) 5131795..5132394 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T203863 WP_002882817.1 NZ_CP075552:c5128143-5127760 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T203863 NZ_CP100428:2616070-2616279 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACATCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT203863 WP_004150355.1 NZ_CP075552:c5128385-5128143 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT203863 NZ_CP100428:2615765-2616058 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References