Detailed information of TA system TA203862

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4778567..4780248
• Replicon: chromosome
• Accession: NZ_CP075552
• Organism: Klebsiella pneumoniae strain RGF99-1

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: -
• Locus tag: KI681_RS23130
• Protein ID: WP_048269735.1
• Coordinates: 4778567..4779484 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A6TH05
• Locus tag: KI681_RS23135
• Protein ID: WP_004177809.1
• Coordinates: 4779490..4780248 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KI681_RS23110 (KI681_23105)	4773873..4775393	+	1521	WP_002885173.1	sugar ABC transporter ATP-binding protein	-
KI681_RS23115 (KI681_23110)	4775390..4776388	+	999	WP_004186549.1	ribose ABC transporter permease	-
KI681_RS23120 (KI681_23115)	4776419..4777360	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
KI681_RS23125 (KI681_23120)	4777435..4778442	+	1008	WP_048269736.1	carbohydrate kinase family protein	-
KI681_RS23130 (KI681_23125)	4778567..4779484	-	918	WP_048269735.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
KI681_RS23135 (KI681_23130)	4779490..4780248	-	759	WP_004177809.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
KI681_RS23140 (KI681_23135)	4780334..4781062	-	729	WP_004177810.1	response regulator transcription factor	-
KI681_RS23145 (KI681_23140)	4781335..4783482	+	2148	WP_077598921.1	formate dehydrogenase subunit alpha	-
KI681_RS23150 (KI681_23145)	4783712..4784398	+	687	WP_004151733.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34646.10 Da
• Isoelectric Point: 5.4746

>T203862 WP_048269735.1 NZ_CP075552:c4779484-4778567 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALERQH
PRDFYDVKLLLDAQELDRPIFNGFIAYLLSHNRPLAEVLSPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T203862 NZ_CP100428:2304463-2304729 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28548.92 Da
• Isoelectric Point: 10.0870

>AT203862 WP_004177809.1 NZ_CP075552:c4780248-4779490 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT203862 NZ_CP100428:2304212-2304463 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAGTAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Antitoxin

• AlphaFold DB: A6TH05