Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4506787..4507597 Replicon chromosome
Accession NZ_CP075552
Organism Klebsiella pneumoniae strain RGF99-1

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag KI681_RS21890 Protein ID WP_002887280.1
Coordinates 4506787..4507320 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KI681_RS21895 Protein ID WP_002887278.1
Coordinates 4507331..4507597 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KI681_RS21885 (KI681_21880) 4505618..4506739 + 1122 WP_009309849.1 cupin domain-containing protein -
KI681_RS21890 (KI681_21885) 4506787..4507320 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
KI681_RS21895 (KI681_21890) 4507331..4507597 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KI681_RS21900 (KI681_21895) 4507700..4509133 - 1434 WP_019724905.1 Cu(+)/Ag(+) sensor histidine kinase -
KI681_RS21905 (KI681_21900) 4509123..4509806 - 684 WP_004214143.1 copper response regulator transcription factor CusR -
KI681_RS21910 (KI681_21905) 4509978..4511360 + 1383 WP_019724904.1 efflux transporter outer membrane subunit -
KI681_RS21915 (KI681_21910) 4511378..4511722 + 345 WP_025987660.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T203861 WP_002887280.1 NZ_CP075552:c4507320-4506787 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T203861 NZ_CP100428:2246016-2246258 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCGGCGAAGTTAGATTTAAAGAAGACATTTATAGAAATTGTTGAGACTTT
AAAAAATAATCCTTATAAAATAACACAATCCTTTGAAAAATTAGAACCTAAATATTTAGAGCGATATTCAAGAAGAATTA
ACCTTCAGCACAGGGTCGTCTATACCATAGATGATCGAAATAAAGTAGTATTAATACTATCGGCATGGTCACATTATGAT
TAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT203861 WP_002887278.1 NZ_CP075552:c4507597-4507331 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT203861 NZ_CP100428:2245759-2246016 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGTAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATGT
ATCTTGTATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References