Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 103476..105099 Replicon plasmid pRGT40-1-tmexCD
Accession NZ_CP075551
Organism Klebsiella pneumoniae strain RGT40-1

Toxin (Protein)


Gene name hipA Uniprot ID C1IUI3
Locus tag KI675_RS27825 Protein ID WP_000088044.1
Coordinates 103476..104801 (-) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A6M3PBR7
Locus tag KI675_RS27830 Protein ID WP_012477377.1
Coordinates 104806..105099 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KI675_RS27795 (KI675_27790) 99462..100319 - 858 WP_000784387.1 HNH endonuclease signature motif containing protein -
KI675_RS27800 (KI675_27795) 100335..100643 - 309 WP_001224686.1 hypothetical protein -
KI675_RS27805 (KI675_27800) 101192..101617 - 426 WP_000278471.1 hypothetical protein -
KI675_RS27810 (KI675_27805) 101870..102685 - 816 WP_001641519.1 HNH endonuclease signature motif containing protein -
KI675_RS27815 (KI675_27810) 102698..103108 - 411 WP_000985909.1 hypothetical protein -
KI675_RS27820 (KI675_27815) 103209..103415 - 207 WP_000134171.1 hypothetical protein -
KI675_RS27825 (KI675_27820) 103476..104801 - 1326 WP_000088044.1 type II toxin-antitoxin system HipA family toxin Toxin
KI675_RS27830 (KI675_27825) 104806..105099 - 294 WP_012477377.1 helix-turn-helix transcriptional regulator Antitoxin
KI675_RS27835 (KI675_27830) 105525..106529 - 1005 WP_000427623.1 IS110-like element IS4321 family transposase -
KI675_RS27840 (KI675_27835) 106608..107042 - 435 WP_003131969.1 mercury resistance transcriptional regulator MerR -
KI675_RS27845 (KI675_27840) 107114..107464 + 351 WP_003131974.1 mercuric ion transporter MerT -
KI675_RS27850 (KI675_27845) 107477..107752 + 276 WP_003131987.1 mercury resistance system periplasmic binding protein MerP -
KI675_RS27855 (KI675_27850) 107824..109509 + 1686 WP_003156770.1 mercury(II) reductase -
KI675_RS27860 (KI675_27855) 109527..109892 + 366 WP_000995360.1 mercury resistance co-regulator MerD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(6)-Id / aph(3'')-Ib / aph(3')-Ia / mph(E) / msr(E) / sul1 / blaDHA-1 / qnrB4 / aac(3)-IId / blaTEM-1B - 1..310767 310767
- flank IS/Tn aph(6)-Id / aph(3'')-Ib - 105525..111857 6332


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(8-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50333.32 Da        Isoelectric Point: 6.5044

>T203848 WP_000088044.1 NZ_CP075551:c104801-103476 [Klebsiella pneumoniae]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T203848 NZ_CP100393:20297-20653 [Aeromonas caviae]
ATGACAAACAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCCACAAGGATTTGATGGCGTTGCCGTCCGACGTGCGTCG
CCGTTTCGGTTACGCGCTCTCGTTGGCGCAGATAGGCGATCAGGATGACGCAGCAAAGGAGCTCAAGGGGTTCGGTGGTG
CCGGCGTGCTGAAGGTCGTCGAAGACGATGCCGGCGGCACCTATCGAGCGGTATACACGGTCAAGTTTGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGAATCGCCACGCCAAAGGCCGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGTATTGGCACAGGAGCTATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10860.30 Da        Isoelectric Point: 10.3134

>AT203848 WP_012477377.1 NZ_CP075551:c105099-104806 [Klebsiella pneumoniae]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW

Download         Length: 294 bp

>AT203848 NZ_CP100393:20655-20990 [Aeromonas caviae]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAGCGCAGCTCGGGCAACGTCTTTGCCGACCTTGGACTGCCTGACGC
TGAAAAGCTCAAGATCAAGACCGGCCTGGTGGTCGAGATCAGGAGGGCCATGCGCGCCCTTGGGCTGACTCAACAAGCGG
CGGCCAAACGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCGCGGCGACTTCACCAATCTATCCGAACGCAAG
CTGATGGATTGTCTGAATCGCCTCGGCTACGACATCGAAATCAAGGTACGGCCAGCAGCCGAGCCGATCGGGCATCTAAC
GCTCGCAACCGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S1XXW1

Antitoxin

Source ID Structure
AlphaFold DB A0A6M3PBR7

References