Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 15708..16618 Replicon plasmid pRGT40-1-tmexCD
Accession NZ_CP075551
Organism Klebsiella pneumoniae strain RGT40-1

Toxin (Protein)


Gene name - Uniprot ID A0A5Q9LR54
Locus tag KI675_RS27390 Protein ID WP_004181896.1
Coordinates 16148..16618 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A2J4RLY9
Locus tag KI675_RS27385 Protein ID WP_004181895.1
Coordinates 15708..16151 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KI675_RS27360 (KI675_27360) 10863..11078 - 216 Protein_12 IS200/IS605 family transposase -
KI675_RS27365 (KI675_27365) 11080..11649 + 570 Protein_13 transposase -
KI675_RS27370 (KI675_27370) 12341..13828 + 1488 WP_004178082.1 group II intron reverse transcriptase/maturase -
KI675_RS27375 (KI675_27375) 13914..14336 + 423 WP_004181893.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
KI675_RS27380 (KI675_27380) 14336..15607 + 1272 WP_004181894.1 translesion error-prone DNA polymerase V subunit UmuC -
KI675_RS27385 (KI675_27385) 15708..16151 + 444 WP_004181895.1 DUF2384 domain-containing protein Antitoxin
KI675_RS27390 (KI675_27390) 16148..16618 + 471 WP_004181896.1 RES family NAD+ phosphorylase Toxin
KI675_RS27395 (KI675_27395) 16728..16988 - 261 WP_004026352.1 hypothetical protein -
KI675_RS27400 (KI675_27400) 17711..17869 + 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
KI675_RS27405 (KI675_27405) 18472..19446 + 975 WP_040222313.1 hypothetical protein -
KI675_RS27410 (KI675_27410) 19644..20021 - 378 WP_004181901.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(6)-Id / aph(3'')-Ib / aph(3')-Ia / mph(E) / msr(E) / sul1 / blaDHA-1 / qnrB4 / aac(3)-IId / blaTEM-1B - 1..310767 310767


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17549.81 Da        Isoelectric Point: 4.6155

>T203847 WP_004181896.1 NZ_CP075551:16148-16618 [Klebsiella pneumoniae]
VIFYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T203847 NZ_CP100392:2585865-2587175 [Aeromonas caviae]
ATGGCCAGCCTGACGGTCTACATGAACGGATACCGGGTAGGTACCTTTACCAAGGCGACCAATGGTGCTCACCAATTTCA
GTACGATGAATCGTGGGTGAAGTTGCCAGGCAGCCGCCCCATCTCGCTCTCCATGCCATTACGCCAGCAGCGCTACCAGG
GCGATGAGGCCTATAACTTCTTCGATAATCTGCTGCCAGATAACCCGGAAGTAAGAAACCGGGTGGTGGCGCGCTATCAA
GCCGATTCCACCCAACCCTTTGATCTGCTCAGTTGCATTGGCCAGGACAGCGTGGGGGCATTGCAACTGGTGGCTCAGGG
CAGACCTGTGCCAGACGTTAAGCGCATTGAGTGCAAGCCGCTCTCTGACGCCGAACTGGAGCAGATCCTGACCAGCTACC
AGCAAGGGATCCCGCTTGGTATGGTGAGGGAAGAGGACGATTTTCGGATATCCATCGCCGGGGCTCAGGAAAAGACCGCG
TTACTCTACCTCGACAACCGTTGGTGCTTACCGCATGCAGCGACCCCAACCACCCACATTATCAAGCTGCCCATTGGCAA
GATCGAAAGCCACTCCTACTCGATAGACCTCTCCCAGAGCGTAGAAAACGAATATCTCTGCACCCTGATCGCCAAAGCAT
TGGGTCTTCCGGTTCCCCATTGCTTCATCATGCAGGTCGGCAAGGTCAAGGCTCTGGCGGTGGAACGCTTCGATCGGCGC
TACGCCACGGATCGCAGTTGGATCATGCGCCTGCCCCAAGAGGACTTCTGCCAGGTGTTGAATGTACCGTCAGCACGCAA
GTACGAGAGCCATGGTGGACCGGGCATTGCTGACATCATGACGACCCTGCTGGGATCGGCCACGCCGGAGCAGGATCGGT
ACCTGTTTATGAAGTCTCAGGTGTTGTTCTGGCTGCTGGCTGCCACTGATGGCCATGCCAAGAATTTCTCGGTGTTTATC
GAACCTGAAGGGCGTTTCCGGCTTACACCGTTTTACGACATCCTTTCCATGTATCCTGCATTTGGTGGTAAAGGATTACA
TCCCCGTGATGCCAAGTTGGCCATGGGGCTTACTGCAACCAAGGGCAAAAAATACGCGATAGAGCAGATATTTCCTCGCC
ATTTTTATCAAACGGCCAAGACGATTGGTTTTGAACAAGTGCAGATGGAAAAAATCATCAGCGAGCTGACGGATAATCTG
GATGAGGTAATATCAATCGTTCGTCAGCAATTGCCTGATACATTTCCCACTGAGATTGCAGATTCAATTTTGGGTGGACT
CAGTTCGAGAGCTCAACGCTTAAAACGATAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16536.87 Da        Isoelectric Point: 10.3013

>AT203847 WP_004181895.1 NZ_CP075551:15708-16151 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT203847 NZ_CP100392:2585611-2585865 [Aeromonas caviae]
ATGAAAGTAACCAGCGCCAAGCAACTTAGTGCCTGTCTCAAGGATGCCAGGCTTTCGCAAAAGCTCTCCCAAGGCAAAGT
GGCCGGCAAGGTGGGGATCAGGCAGGACACGGTGTCCAGCTTCGAGCTCAATCCCGAGTCAACCAAGCTGGAGACCTTGT
TCAAGATCCTCGCCGCACTGGAACTCTCCCTCGATATCAGGCCCCGCACCGATCCTGCGGAAAACGCCACGCCTGGCTGG
AAGGAGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q9LR54

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4RLY9

References