Detailed information of TA system TA203846

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 55896..56539
• Replicon: plasmid pRGT40-1-78k
• Accession: NZ_CP075550
• Organism: Klebsiella pneumoniae strain RGT40-1

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: KI675_RS27175
• Protein ID: WP_001044770.1
• Coordinates: 55896..56312 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: KI675_RS27180
• Protein ID: WP_001261282.1
• Coordinates: 56309..56539 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KI675_RS27155 (KI675_27155)	51077..52045	+	969	WP_000654802.1	IS5-like element IS903B family transposase	-
KI675_RS27160 (KI675_27160)	52101..52715	-	615	Protein_55	HNH endonuclease	-
KI675_RS27165 (KI675_27165)	53253..54275	-	1023	WP_000361404.1	helicase UvrD	-
KI675_RS27170 (KI675_27170)	54260..55822	-	1563	WP_004206609.1	AAA family ATPase	-
KI675_RS27175 (KI675_27175)	55896..56312	-	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
KI675_RS27180 (KI675_27180)	56309..56539	-	231	WP_001261282.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KI675_RS27185 (KI675_27185)	56496..56957	+	462	WP_074193041.1	hypothetical protein	-
KI675_RS27190 (KI675_27190)	57116..57409	+	294	WP_020956883.1	hypothetical protein	-
KI675_RS27195 (KI675_27195)	57473..58159	+	687	WP_020956882.1	hypothetical protein	-
KI675_RS27200 (KI675_27200)	58171..58947	+	777	WP_020956881.1	tyrosine-type recombinase/integrase	-
KI675_RS27205 (KI675_27205)	59005..59262	-	258	WP_000764642.1	hypothetical protein	-
KI675_RS27210 (KI675_27210)	59391..59495	-	105	WP_032409716.1	hypothetical protein	-
KI675_RS27215 (KI675_27215)	60025..60891	+	867	WP_004118283.1	replication initiation protein	-
KI675_RS27220 (KI675_27220)	61068..61337	-	270	WP_000339857.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	sul2 / tet(A) / blaLAP-2 / qnrS1 / aadA2 / dfrA12	-	1..78533	78533
	-	flank	IS/Tn	-	-	51122..52045	923

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T203846 WP_001044770.1 NZ_CP075550:c56312-55896 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T203846 NZ_CP100391:c3442183-3441833 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT203846 WP_001261282.1 NZ_CP075550:c56539-56309 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT203846 NZ_CP100391:c3442470-3442189 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3