Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 65504..66081 Replicon plasmid pRGT40-1-181k
Accession NZ_CP075549
Organism Klebsiella pneumoniae strain RGT40-1

Toxin (Protein)


Gene name mazF Uniprot ID A0A7G3NNX6
Locus tag KI675_RS26200 Protein ID WP_059065587.1
Coordinates 65749..66081 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A7G3NP82
Locus tag KI675_RS26195 Protein ID WP_032454911.1
Coordinates 65504..65749 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KI675_RS26155 (KI675_26155) 61399..61974 - 576 WP_094313438.1 hypothetical protein -
KI675_RS26160 (KI675_26160) 62109..62363 - 255 Protein_63 IS1 family transposase -
KI675_RS26165 (KI675_26165) 62450..62563 + 114 Protein_64 helix-turn-helix domain-containing protein -
KI675_RS26170 (KI675_26170) 62683..63063 + 381 Protein_65 transposase family protein -
KI675_RS26175 (KI675_26175) 63065..63259 + 195 Protein_66 colicin E3/pyocin S6 family cytotoxin -
KI675_RS26180 (KI675_26180) 63269..63526 + 258 WP_060655703.1 cloacin immunity family protein -
KI675_RS26185 (KI675_26185) 63723..64415 - 693 WP_094313752.1 Exc2 family lipoprotein -
KI675_RS26190 (KI675_26190) 64464..64799 - 336 WP_170933330.1 TonB family protein -
KI675_RS26195 (KI675_26195) 65504..65749 + 246 WP_032454911.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
KI675_RS26200 (KI675_26200) 65749..66081 + 333 WP_059065587.1 endoribonuclease MazF Toxin
KI675_RS26205 (KI675_26205) 66146..66238 + 93 Protein_72 IS3 family transposase -
KI675_RS26210 (KI675_26210) 66675..68084 - 1410 Protein_73 group II intron reverse transcriptase/maturase -
KI675_RS26215 (KI675_26215) 68127..69314 - 1188 Protein_74 ISL3-like element ISCfr12 family transposase -
KI675_RS26220 (KI675_26220) 69432..69881 - 450 WP_105910642.1 AfaD family invasin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / sul2 / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / aac(3)-IId / aac(6')-Ib-cr / ARR-3 / dfrA27 / aadA16 / qacE / sul1 / mph(A) iutA / iucD / iucC / iucB / iucA / cseA 1..181365 181365


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(9-107)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11991.87 Da        Isoelectric Point: 7.7494

>T203845 WP_059065587.1 NZ_CP075549:65749-66081 [Klebsiella pneumoniae]
MVSRFVPDAGDLIWINFDPVEGHEQGGHRPAVVLSPFAYNNKTGLLLCVPCTTKVKGYPFEVELPGERDGVALADQITCV
DWRARKVEKKGKVATGELSEIRAKAKALIG

Download         Length: 333 bp

>T203845 NZ_CP100391:2690313-2691059 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTGAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCTGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8881.21 Da        Isoelectric Point: 5.7996

>AT203845 WP_032454911.1 NZ_CP075549:65504-65749 [Klebsiella pneumoniae]
MSQATFKKWGNQAGIRFPAAVMKAAALNVDDTVDIEVQEGRIVLIPTKEKTYSLDALLSGITEENMHNKADFGKPTGKEM
L

Download         Length: 246 bp

>AT203845 NZ_CP100391:2691060-2691230 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7G3NNX6

Antitoxin

Source ID Structure
AlphaFold DB A0A7G3NP82

References