Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 52612..53255 Replicon plasmid pRGT40-1-181k
Accession NZ_CP075549
Organism Klebsiella pneumoniae strain RGT40-1

Toxin (Protein)


Gene name vagD Uniprot ID A0A6H2GRP8
Locus tag KI675_RS26110 Protein ID WP_023292131.1
Coordinates 52839..53255 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A6B7Q5S4
Locus tag KI675_RS26105 Protein ID WP_021312476.1
Coordinates 52612..52842 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KI675_RS26080 (KI675_26080) 47679..48856 + 1178 Protein_46 IS3 family transposase -
KI675_RS26085 (KI675_26085) 49238..50023 - 786 WP_050485696.1 site-specific integrase -
KI675_RS26090 (KI675_26090) 50069..50590 - 522 WP_094313782.1 hypothetical protein -
KI675_RS26095 (KI675_26095) 50648..51040 - 393 WP_170933338.1 hypothetical protein -
KI675_RS26100 (KI675_26100) 51077..52021 - 945 WP_094313783.1 hypothetical protein -
KI675_RS26105 (KI675_26105) 52612..52842 + 231 WP_021312476.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KI675_RS26110 (KI675_26110) 52839..53255 + 417 WP_023292131.1 type II toxin-antitoxin system VapC family toxin Toxin
KI675_RS26115 (KI675_26115) 53290..53535 + 246 WP_065801591.1 hypothetical protein -
KI675_RS26120 (KI675_26120) 53599..53853 + 255 WP_095153353.1 hypothetical protein -
KI675_RS26125 (KI675_26125) 54410..55416 + 1007 Protein_55 IS110 family transposase -
KI675_RS29145 55695..55943 + 249 WP_065801593.1 hypothetical protein -
KI675_RS26130 (KI675_26130) 55948..56322 + 375 Protein_57 hypothetical protein -
KI675_RS26135 (KI675_26135) 56301..57005 - 705 Protein_58 UxaA family hydrolase -
KI675_RS26140 (KI675_26140) 57010..57303 + 294 Protein_59 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / sul2 / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / aac(3)-IId / aac(6')-Ib-cr / ARR-3 / dfrA27 / aadA16 / qacE / sul1 / mph(A) iutA / iucD / iucC / iucB / iucA / cseA 1..181365 181365


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14975.40 Da        Isoelectric Point: 8.5464

>T203844 WP_023292131.1 NZ_CP075549:52839-53255 [Klebsiella pneumoniae]
VKKTFMLDTNICSFIMREQPAAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHIELVDAFCARLDAILPWDRA
AVDATTEIKVALRQAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLVLEDWVN

Download         Length: 417 bp

>T203844 NZ_CP100390:c2161060-2160575 [Alkalimarinus sp. A2M4]
ATGGGAAGAATAAGTAAGCCGGAACCCCTAAGGTCAGTACACGACCTTAGCAGATTTAGCTGTGGCATCGATTCACTTGA
TCAATGGTTGAAAACCAGAGCCCTAAAAAATGAAAGAATCAATGCTTCCAGAACATATGTAGTATGTGATGAGCTTCGTG
TGGCTGGATATTATTGTTTGGCGACAGGTTCAATAGAACATAATGAAAGTCCTAGCAAAGTAAAAAGGAATATGCCTTCA
CCGATTCCAGTCATGATACTAGGGCGGTTGGCTGTAGATATAGATTATCAAGACCAAAAAATTGGCAAAGGTCTATTGAA
AGATGCTGTATTGCGAACAATTTTGATCTCAGAGCAAGTTGGGGTTAAAGCCATGCTAGTACACGCAATCAATGATAGTG
CTCGATATTTCTATAAAGCACATGGATTCATAGAAAGTCCATTCGATCCAATGAAACTATTATTGCCAACTGCAAAGAAG
GTATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8723.76 Da        Isoelectric Point: 4.3410

>AT203844 WP_021312476.1 NZ_CP075549:52612-52842 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFDL

Download         Length: 231 bp

>AT203844 NZ_CP100390:c2161323-2161048 [Alkalimarinus sp. A2M4]
ATGACTAAGGCAATACATAAAGTACCGATAAACATGAGAGCTGATGAAGATCTGAGAAACCTGATTGATCAAGCAGCAGC
AATTTCTAGTAAAGATAGAACTAGCTTTATCCTCGAAACCATGAGGCGGGAGTCTGAGAATATTATCCTTGATCAGAGAC
TGTTTAAATTAGACGAAGAGGCATTTTCTCAACTTGATGAATTGATCAGTGTGCCTATTTCCTCTAACCCGGCTTTAACA
AAACTATTATCTTCAAAATCTCCATGGGAAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6H2GRP8

Antitoxin

Source ID Structure
AlphaFold DB A0A6B7Q5S4

References