Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5241170..5241795 Replicon chromosome
Accession NZ_CP075548
Organism Klebsiella pneumoniae strain RGT40-1

Toxin (Protein)


Gene name vapC Uniprot ID A0A483GDF6
Locus tag KI675_RS25505 Protein ID WP_004187928.1
Coordinates 5241170..5241553 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KI675_RS25510 Protein ID WP_004150355.1
Coordinates 5241553..5241795 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KI675_RS25490 (5238536) 5238536..5239438 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KI675_RS25495 (5239435) 5239435..5240070 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KI675_RS25500 (5240067) 5240067..5240996 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KI675_RS25505 (5241170) 5241170..5241553 - 384 WP_004187928.1 type II toxin-antitoxin system VapC family toxin Toxin
KI675_RS25510 (5241553) 5241553..5241795 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KI675_RS25515 (5241989) 5241989..5242906 + 918 WP_004187929.1 alpha/beta hydrolase -
KI675_RS25520 (5242920) 5242920..5243861 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KI675_RS25525 (5243906) 5243906..5244343 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KI675_RS25530 (5244340) 5244340..5245200 - 861 WP_004146232.1 virulence factor BrkB family protein -
KI675_RS25535 (5245194) 5245194..5245793 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14407.64 Da        Isoelectric Point: 7.3178

>T203843 WP_004187928.1 NZ_CP075548:c5241553-5241170 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFTGIPGVVTPYEIHPE

Download         Length: 384 bp

>T203843 NZ_CP100390:c1350506-1350297 [Alkalimarinus sp. A2M4]
ATACCTGATGTAGTTGAAGTTGGGCACGTGATTAAACTATTACAACTTGGTGAAACTTTGCCAGATAAATATGTTGATCA
TTTGTTGTCGGGTAATTGGCAAGGTTACCGTGATTGTCATGTTAAGCCAGATTTAGTTTTGATATATAAAGTAGATAACA
ACTCTTTAAAACTTGCAAGGATTGGAACTCATAGTGAGCTATTTCGCTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT203843 WP_004150355.1 NZ_CP075548:c5241795-5241553 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT203843 NZ_CP100390:c1350842-1350570 [Alkalimarinus sp. A2M4]
ATGAAGACAGAAATGCTAAGTACACGCATAGACCATGACACAAAGCTAGCATTTACTAATGTATGTGACGATATGGGTCT
AAGCACCTCGCAAGCCTTAAAGCTATTTGCCAAAGCTGTTATTAATTATGGTGGAATACCTTTTGAACTTAAAGCAAAAA
CACCAAATACCTTGACAGCAAATGCAATTGAAGAGCTAGAGTCTGGAAATGGCACTAAAGCTGGAACGATTGAAGAGCTT
TTTAACCATTTAGACATTGATGGCTTGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483GDF6

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References