Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4635534..4636344 Replicon chromosome
Accession NZ_CP075548
Organism Klebsiella pneumoniae strain RGT40-1

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag KI675_RS22645 Protein ID WP_004178461.1
Coordinates 4635534..4636067 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KI675_RS22650 Protein ID WP_002887278.1
Coordinates 4636078..4636344 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KI675_RS22640 (4634365) 4634365..4635486 + 1122 WP_016946712.1 cupin domain-containing protein -
KI675_RS22645 (4635534) 4635534..4636067 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
KI675_RS22650 (4636078) 4636078..4636344 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KI675_RS22655 (4636447) 4636447..4637880 - 1434 WP_048984453.1 Cu(+)/Ag(+) sensor histidine kinase -
KI675_RS22660 (4637870) 4637870..4638553 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
KI675_RS22665 (4638726) 4638726..4640111 + 1386 WP_040238382.1 efflux transporter outer membrane subunit -
KI675_RS22670 (4640129) 4640129..4640473 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T203839 WP_004178461.1 NZ_CP075548:c4636067-4635534 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T203839 NZ_CP100390:1347406-1347660 [Alkalimarinus sp. A2M4]
ATGAGAGTTGTATCTTTTACTGAAGCAAGAAATGGATTAAAAGCTGTTTTAGATTCTGTCGTTAACGATGCAGATACCAC
CGTTATAACGCGCAGAGACTCAGAGGACGCTGTGGTAATGTCTTTAGATTACTACAATAGTTTAATGGAGACTGTTCATT
TGTTAAGGTCTCCTGCAAATGCCGAGCATCTAAGTAAATCTATAGCGCAATACAAAAGTGGCCAAACAACTCAAAGAGAA
ATAATTGATGAGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT203839 WP_002887278.1 NZ_CP075548:c4636344-4636078 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT203839 NZ_CP100390:1347653-1347916 [Alkalimarinus sp. A2M4]
ATGAGTAGCAGGTTGCTTAGTTGGACAGATGAAGCGTGGAATGACTATATTTATTGGCAAGGTCAGGATAAAAAGACTTT
AAAACGTATCAATAAATTAATTAGTGACACAATGCGCTCGCCATTTGAGGGTATAGGTAAACCTGAACCTCTTAAAGAAA
ATTTGTCAGGTTTCTGGTCTCGTAGAATAGATGATACAAATAGGTTAGTGTATGTAGTCAATGACAAAGCATTGACCATA
ATTTCGTGCAGGTATCATTATTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References