Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4037023..4037642 Replicon chromosome
Accession NZ_CP075548
Organism Klebsiella pneumoniae strain RGT40-1

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KI675_RS19785 Protein ID WP_002892050.1
Coordinates 4037424..4037642 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KI675_RS19780 Protein ID WP_002892066.1
Coordinates 4037023..4037397 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KI675_RS19770 (4032175) 4032175..4033368 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KI675_RS19775 (4033391) 4033391..4036537 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
KI675_RS19780 (4037023) 4037023..4037397 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KI675_RS19785 (4037424) 4037424..4037642 + 219 WP_002892050.1 HHA domain-containing protein Toxin
KI675_RS19790 (4037805) 4037805..4038371 + 567 WP_002892030.1 maltose O-acetyltransferase -
KI675_RS19795 (4038343) 4038343..4038483 - 141 WP_004147370.1 hypothetical protein -
KI675_RS19800 (4038504) 4038504..4038974 + 471 WP_002892026.1 YlaC family protein -
KI675_RS19805 (4038949) 4038949..4040400 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
KI675_RS19810 (4040501) 4040501..4041199 + 699 WP_002892021.1 GNAT family protein -
KI675_RS19815 (4041196) 4041196..4041336 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KI675_RS19820 (4041336) 4041336..4041599 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T203838 WP_002892050.1 NZ_CP075548:4037424-4037642 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T203838 NZ_CP100390:c1343013-1342768 [Alkalimarinus sp. A2M4]
ATGACCTATGAGCTAGAGTTTAAAAAGTCTGCCCTGAAAGAGTGGAATAAACTAGGCGCTACGGTAAAAGAACAATTTAA
GAAAAAGCTAGCCGCAGTTTTAGATAGCCCCAAAATTAAAAGCGCCAAACTATCAGGGGCAAACGAGCTTTATAAAATTA
AGCTAAGGCAGGCTGGATATAGATTAGTTTATGAAGTTAATGATGAAATAGTAATAGTTACCGTGATATCTGTGGGAAAA
CGATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT203838 WP_002892066.1 NZ_CP075548:4037023-4037397 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT203838 NZ_CP100390:c1343251-1343003 [Alkalimarinus sp. A2M4]
ATGACAACGCGAATTTTAACTGAAATGGCTGCAAGCATAAGCGAGCTCAAAGCAAATCCAATGAGAGTAGCACTCAGCGG
TGACGGTGAGCCAATAGCTGTACTGAATAGAAATGAGCCTGCATTTTATTGTATACCTGCTGAAACCTACGAATACTTAA
TGGATAGAATTGAAGATCTCGAGCTTATTACACTTGCTGAGAAACGGAAAAGTGAAGAAAGTGTAAAGGTTAATTTGAAT
GACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References