Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/MazF(toxin) |
| Location | 475830..476467 | Replicon | chromosome |
| Accession | NZ_CP075547 | ||
| Organism | Bacillus amyloliquefaciens strain SN16-1 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | G4NU33 |
| Locus tag | KHS93_RS02440 | Protein ID | WP_003156187.1 |
| Coordinates | 476117..476467 (+) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | I2HMS5 |
| Locus tag | KHS93_RS02435 | Protein ID | WP_003156188.1 |
| Coordinates | 475830..476111 (+) | Length | 94 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KHS93_RS02415 (KHS93_02415) | 472195..472794 | - | 600 | WP_003156193.1 | rhomboid family intramembrane serine protease | - |
| KHS93_RS02420 (KHS93_02420) | 472887..473252 | + | 366 | WP_003156192.1 | holo-ACP synthase | - |
| KHS93_RS02425 (KHS93_02425) | 473417..474424 | + | 1008 | WP_003156191.1 | outer membrane lipoprotein carrier protein LolA | - |
| KHS93_RS02430 (KHS93_02430) | 474541..475710 | + | 1170 | WP_003156189.1 | alanine racemase | - |
| KHS93_RS02435 (KHS93_02435) | 475830..476111 | + | 282 | WP_003156188.1 | type II toxin-antitoxin system antitoxin EndoAI | Antitoxin |
| KHS93_RS02440 (KHS93_02440) | 476117..476467 | + | 351 | WP_003156187.1 | type II toxin-antitoxin system endoribonuclease NdoA | Toxin |
| KHS93_RS02445 (KHS93_02445) | 476585..477406 | + | 822 | WP_003156182.1 | STAS domain-containing protein | - |
| KHS93_RS02450 (KHS93_02450) | 477411..477776 | + | 366 | WP_003156180.1 | RsbT antagonist protein RsbS | - |
| KHS93_RS02455 (KHS93_02455) | 477779..478180 | + | 402 | WP_003156178.1 | anti-sigma regulatory factor | - |
| KHS93_RS02460 (KHS93_02460) | 478192..479199 | + | 1008 | WP_003156177.1 | PP2C family protein-serine/threonine phosphatase | - |
| KHS93_RS02465 (KHS93_02465) | 479263..479592 | + | 330 | WP_003156176.1 | anti-sigma factor antagonist RsbV | - |
| KHS93_RS02470 (KHS93_02470) | 479589..480071 | + | 483 | WP_161503396.1 | anti-sigma B factor RsbW | - |
| KHS93_RS02475 (KHS93_02475) | 480037..480825 | + | 789 | WP_003156171.1 | RNA polymerase sigma factor SigB | - |
| KHS93_RS02480 (KHS93_02480) | 480825..481427 | + | 603 | WP_003156170.1 | PP2C family serine/threonine-protein phosphatase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 12977.98 Da Isoelectric Point: 4.8781
>T203822 WP_003156187.1 NZ_CP075547:476117-476467 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
Download Length: 351 bp
>T203822 NZ_CP100388:c303436-303149 [Enterobacter hormaechei subsp. steigerwaltii]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGGTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTGAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCAGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTGGGTTAACAATCAAACCCTCTGGGGATTAA
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGGTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTGAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCAGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTGGGTTAACAATCAAACCCTCTGGGGATTAA
Antitoxin
Download Length: 94 a.a. Molecular weight: 10562.92 Da Isoelectric Point: 6.4706
>AT203822 WP_003156188.1 NZ_CP075547:475830-476111 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG
Download Length: 282 bp
>AT203822 NZ_CP100388:c303745-303443 [Enterobacter hormaechei subsp. steigerwaltii]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGAATCAGTGAACTGAGGATCC
ACTTTGGCCCGGGCTACAGAGTCTATTTTAAGGACCAGGGCAATTGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGAATCAGTGAACTGAGGATCC
ACTTTGGCCCGGGCTACAGAGTCTATTTTAAGGACCAGGGCAATTGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|