Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4174961..4175458 Replicon chromosome
Accession NZ_CP075487
Organism Escherichia coli strain NT1F34

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag IGI26_RS20605 Protein ID WP_001173343.1
Coordinates 4174961..4175134 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag IGI26_RS20610 Protein ID WP_000398619.1
Coordinates 4175162..4175458 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI26_RS20595 (4171631) 4171631..4173088 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
IGI26_RS20600 (4173325) 4173325..4174842 + 1518 WP_001295074.1 lysine--tRNA ligase -
IGI26_RS20605 (4174961) 4174961..4175134 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
IGI26_RS20610 (4175162) 4175162..4175458 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
IGI26_RS20615 (4175685) 4175685..4175957 - 273 WP_073544413.1 GNAT family N-acetyltransferase -
IGI26_RS20620 (4175969) 4175969..4176199 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
IGI26_RS20625 (4176380) 4176380..4178011 + 1632 WP_001216477.1 sensor histidine kinase -
IGI26_RS20630 (4178008) 4178008..4178727 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4155218..4175458 20240


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T203819 WP_001173343.1 NZ_CP075487:c4175134-4174961 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T203819 NZ_CP100351:2889503-2889904 [Desulfomicrobium sp. ZS1]
ATGCTCAAATATCTGCTGGACACGAACATCGTCATCTACGTCATCAAGAACCGCCCCCTGTCTGTCCTGAAAACCTTCAA
CCAGAACCACGGCCGCATGGCCATCTCGTCCATAACGCTGGCGGAGCTTGCGCACGGAGTGGAAAAGAGCGCCGATCCGG
CCCGGAACCTGACCGTTGTGGAAGACTTCGTCAGCCGTCTGGAAGTGCTCCCCTACGATGAGCGGGCCGCCTGGCAGTAC
GGCCTCATCCGCGCTGCATTGGAACGGCAAGGCACGCCTATCGGCGTGAATGACCTCCACATCGCGGGACACGCTCGCAG
CCTGGGACTGGTCATCGTCACCAACAACCTGCGGGAATTCGAACGAGTCCCCGGAGTGAGCGTCGAGAATTGGGTGGAAT
AG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT203819 WP_000398619.1 NZ_CP075487:c4175458-4175162 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT203819 NZ_CP100351:2889267-2889500 [Desulfomicrobium sp. ZS1]
ATGGAAACAGGAACCGTATTCATCAACAATCGTACGCAGGCAGTGCGCCTTCCTGCCGGGACTCGATTTCCGGATTCCGT
AAAAAGGGTTCAGGTCAGGGTCGTGGGCAAGGAGCGGATTCTCACCCCTGTCGATGCGGCTTGGGACAGCTTTTTCCTCG
GCGGCCTGACCGTCACCGACGACTTCATGGCCGAACGGGCCGAACAGGCACAGTCAGAACGGGAAGCATTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References