Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3425655..3426273 Replicon chromosome
Accession NZ_CP075487
Organism Escherichia coli strain NT1F34

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag IGI26_RS17090 Protein ID WP_001291435.1
Coordinates 3426055..3426273 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag IGI26_RS17085 Protein ID WP_000344800.1
Coordinates 3425655..3426029 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IGI26_RS17075 (3420744) 3420744..3421937 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
IGI26_RS17080 (3421960) 3421960..3425109 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
IGI26_RS17085 (3425655) 3425655..3426029 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
IGI26_RS17090 (3426055) 3426055..3426273 + 219 WP_001291435.1 HHA domain-containing protein Toxin
IGI26_RS17095 (3426445) 3426445..3426996 + 552 WP_000102564.1 maltose O-acetyltransferase -
IGI26_RS17100 (3427112) 3427112..3427582 + 471 WP_000136192.1 YlaC family protein -
IGI26_RS17105 (3427746) 3427746..3429296 + 1551 WP_023486216.1 cyclic-guanylate-specific phosphodiesterase PdeB -
IGI26_RS17110 (3429338) 3429338..3429691 - 354 WP_000878140.1 DUF1428 domain-containing protein -
IGI26_RS17120 (3430070) 3430070..3430381 + 312 WP_000409911.1 MGMT family protein -
IGI26_RS17125 (3430412) 3430412..3430984 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T203817 WP_001291435.1 NZ_CP075487:3426055-3426273 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T203817 NZ_CP100351:c2689561-2689331 [Desulfomicrobium sp. ZS1]
ATGAGTTATATCGCACGTGATGACGCTGAATTGGCCAGGGAAATATACTGCGAAGTCCGGAGGCGAGCCTCTGAACTGTC
CCATTTCCCGGAGTCCGGTTGTCCTGGGCGTGTCCCCGGGACGAGAGAACTGGTCCTTGCCAGATACCCATACGTACTGC
CCTACCGCGTGAGGACCGGTATCGTTGAAATTTTACGGGTCTTTCACACTAGCCAAAAGATGGATTGCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT203817 WP_000344800.1 NZ_CP075487:3425655-3426029 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT203817 NZ_CP100351:c2689841-2689599 [Desulfomicrobium sp. ZS1]
ATGTCCACCCAAATATCAATCCGCACAAGCGAGGATCTGATCCTCAAATTCAACGCGCTGGCAAAGGAAACTGATCGCTC
TCGCGCCTACCTCATCAACCAGGCGTTGGAGGAATACATTGCGCGTGAGGCGTGGCAGGTTGCCGAAATCCGGAAGGCTC
TCCAGGAAGCTGACGCTGGGGACTTTGCGACGGACGAAGAAATAGCGGCGCTCGATGCGAAGTGGGGCTATCGTGCGGGT
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References